Crowding-induced cooperativity in DNA surface hybridization.
Crowding-induced cooperativity in DNA surface hybridization.
复制标题
DNA 表面杂交中拥挤诱导的协同性
DOI：
10.1038/srep09217
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发表时间：
2015-04-15
影响因子：
4.6
通讯作者：
Ma YQ
中科院分区：
文献类型：
--
作者：
Lei QL;Ren CL;Su XH;Ma YQ
高密度DNA刷不仅用于模拟细胞拥挤，而且在DNA功能化材料中也有广泛的应用。实验表明这些系统中存在复杂的协同杂交/熔化现象，提出了分子拥挤如何影响 DNA 杂交的问题。在这项工作中，提出了一个理论模型，包括所有可能的分子间和分子内相互作用，以及不同物种的分子细节。我们发现分子拥挤可以导致两种不同的合作行为：以更宽的过渡宽度为标志的负合作杂交和以更尖锐的过渡为标志的正合作杂交，这很好地协调了实验结果。此外，还发现了正协同性导致的相变。我们的研究提供了关于细胞拥挤和区隔的新见解，并且在控制 DNA 功能化纳米粒子的表面形态方面具有潜在价值。
High density DNA brush is not only used to model cellular crowding, but also has a wide application in DNA-functionalized materials. Experiments have shown complicated cooperative hybridization/melting phenomena in these systems, raising the question that how molecular crowding influences DNA hybridization. In this work, a theoretical modeling including all possible inter and intramolecular interactions, as well as molecular details for different species, is proposed. We find that molecular crowding can lead to two distinct cooperative behaviours: negatively cooperative hybridization marked by a broader transition width and positively cooperative hybridization with a sharper transition, well reconciling the experimental findings. Moreover, a phase transition as a result of positive cooperativity is also found. Our study provides new insights in crowding and compartmentation in cell and has the potential value in controlling surface morphologies of DNA functionalized nano-particles.