Preferantial localization of type I phosphatidylinositol 4-phosphate 5-kinase γ at the periactive zone of mouse photoreceptor ribbon synapses.
Preferantial localization of type I phosphatidylinositol 4-phosphate 5-kinase γ at the periactive zone of mouse photoreceptor ribbon synapses.
复制标题
I 型磷脂酰肌醇 4-磷酸 5-激酶 γ 在小鼠感光带突触周围活性区的优先定位。
DOI：
10.1016/j.brainres.2014.08.051
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发表时间：
2014
期刊：
影响因子：
2.9
通讯作者：
Fukaya M.
中科院分区：
文献类型：
--
作者：
Sakagami H;Katsumata O;Hara Y;Tamaki H;Fukaya M.
I 型磷脂酰肌醇 4-磷酸 5 激酶 γ (PIP5KIγ) 是生成磷脂酰肌醇 4,5-二磷酸 (PtdIns(4,5)P2) 的主要途径，调节突触前和突触后区室的多种神经元功能。在这项研究中，我们检查了 PIP5KIγ 在成年小鼠视网膜中的表达和定位。 RT-PCR分析显示PIP5KIγ_v2主要在视网膜中表达，而PIP5KIγ_v3也有微弱表达。成年小鼠视网膜的免疫染色显示，内丛状层和外丛状层以点状方式存在强烈的 PIP5KIγ 免疫反应性。在感光带突触中，PIP5KIγ 高度集中在周围活动区。这些发现表明，PIP5KIγ，尤其是 PIP5KIγ_i2，位于周围活性区，这是一个功能上适合感光带突触中突触小泡内吞作用的区室。
Type I phosphatidylinositol 4-phosphate 5 kinase γ (PIP5KIγ) constitutes a major pathway for the generation of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) that regulates a variety of neuronal functions at both presynaptic and postsynaptic compartments. In this study, we examined the expression and localization of PIP5KIγ in the adult mouse retina. RT-PCR analysis revealed that PIP5KIγ_v2 was predominantly expressed in the retina while PIP5KIγ_v3 was also expressed faintly. Immunostaining of the adult mouse retina revealed intense PIP5KIγ-immunoreactivity in the inner and outer plexiform layers in a punctate manner. In the photoreceptor ribbon synapse, PIP5KIγ was highly concentrated at the periactive zone. These findings suggest that PIP5KIγ, especially PIP5KIγ_i2, is localized at the periactive zone, a functionally suitable compartment for the endocytosis of synaptic vesicles in photoreceptor ribbon synapses.