A confocal microscopical study of the musculature of adult Schistosoma mansoni
A confocal microscopical study of the musculature of adult Schistosoma mansoni
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DOI：
10.1017/s0031182099006174
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发表时间：
2000-08-01
期刊：
影响因子：
2.4
通讯作者：
Halton, DW
中科院分区：
文献类型：
--
作者：
Mair, GR;Maule, AG;Halton, DW
使用丝状肌动蛋白标记物，FITC-结合鬼笔环肽，成年男性和女性的主要肌肉系统已被检查。体壁肌肉系统包括圆形纤维的外鞘，在所述外鞘内存在短的纺锤形纵向纤维的紧密层和内部对角纤维的网格状布置。在口吸盘和髋臼内，可区分出3种纤维类型，即圆形、径向和纵向纤维。食管壁由圆形和纵向纤维的网格状阵列构成，而肠盲囊的壁仅包含宽的圆形纤维。在雌性生殖系统中，只有环状纤维存在于输卵管、卵黄管和子宫中。相反，卵型的壁显示紧密排列的圆形和纵向肌纤维。先前鉴定的肌活性化合物（血清素[5-羟色胺，5-HT]，神经肽F [Moniezia expansa]和GYIRFamide [Bdelloura candida，Dugesia tigrina]）的抗血清在特定神经纤维和各种肌肉系统的空间相互关系的初步研究中用作神经元标记物。5-羟色胺能纤维支配这两个吸盘，也构成了一个皮层下的神经网。在雄性中，它们为雌核管的背腹肌纤维提供神经支配，在雌性中，它们为卵型的圆形和纵向肌纤维提供神经支配。神经肽F和FMRFamide相关肽GYIRFamide都位于与妇科管的背腹侧纤维相关的神经丛内，并且在腹侧和口吸盘的神经支配中是明显的。
Using the filamentous actin marker, FITC-conjugated phalloidin, the major muscle systems of adult male and female schistosomes have been examined. The body wall musculature comprises an outer sheath of circular fibres, within which there is a compact layer of short, spindle-shaped longitudinal fibres and a lattice-like arrangement of inner diagonal fibres. Within the Oral sucker and acetabulum 3 fibre types, circular, radial and longitudinal can be distinguished. The wall of the oesophagus is lilted by a grid-like array of circular and longitudinal fibres, whereas the walls of the intestinal caeca contain only comparably broad circular fibres. Within the female reproductive system, only circular fibres are present in the oviduct, vitelline duct and uterus. In contrast, the wall of the ootype displays closely arranged circular and longitudinal muscle fibers. Antisera to previously identified myoactive compounds (serotonin [5-hydroxytryptamine, 5-HT], neuropeptide F [Moniezia expansa] and GYIRFamide [Bdelloura candida, Dugesia tigrina]) were used as neuronal markers in a preliminary study of the spatial inter-relationships of specific nerve fibres and various muscle systems. Serotoninergic fibres innervate both suckers and also constitute a subtegumental nerve net. In males they provide innervation to the dorso-ventral muscle fibres of the gynaecophoric canal, and in females they innervate the circular and longitudinal muscle fibres of the ootype. Neuropeptide F and the FMRFamide-related peptide, GYIRFamide are both localized within nerve plexuses associated with the dorso-ventral fibres of the gynaecophoric canal, and are evident in the innervation of the ventral and oral sucker.