Alu RNP and Alu RNA regulate translation initiation in vitro.
Alu RNP and Alu RNA regulate translation initiation in vitro.
复制标题
Alu RNP和Alu RNA在体外调节翻译起始。
DOI：
10.1093/nar/gkl246
复制
发表时间：
2006
影响因子：
14.9
通讯作者：
Strub, Katharina
中科院分区：
文献类型：
--
作者：
Haesler, Julien;Strub, Katharina
Alu元件是人类基因组中最丰富的重复元件;它们来自信号识别颗粒RNA基因，由两个相关但不同的单体（左臂和右臂）组成。从这些元件转录的Alu RNA在正常细胞生长时以低水平存在，但各种应激条件增加了它们的丰度。已知Alu RNA结合同源蛋白SRP 9/14。我们纯化了合成的Alu RNP，由Alu RNA与SRP 9/14复合组成，并研究了Alu RNP和裸Alu RNA对蛋白质翻译的影响。我们发现，二聚体的Alu RNP和单体的左和右Alu RNP对蛋白质翻译具有一般的剂量依赖性抑制作用。在不存在SRP 9/14的情况下，Alu RNA对所有报告基因mRNA具有刺激作用。sRight RNA的不稳定结构表明Alu RNP和Alu RNA的差异活性可以通过RNA的构象变化来解释。我们证明，Alu RNP和Alu RNA在翻译过程中不稳定地与核糖体相关联，并且基于对多核糖体概况和同步翻译的分析，我们表明Alu RNP和Alu RNA在起始水平上调节翻译。
Alu elements are the most abundant repetitive elements in the human genome; they emerged from the signal recognition particle RNA gene and are composed of two related but distinct monomers (left and right arms). Alu RNAs transcribed from these elements are present at low levels at normal cell growth but various stress conditions increase their abundance. Alu RNAs are known to bind the cognate proteins SRP9/14. We purified synthetic Alu RNP, composed of Alu RNA in complex with SRP9/14, and investigated the effects of Alu RNPs and naked Alu RNA on protein translation. We found that the dimeric Alu RNP and the monomeric left and right Alu RNPs have a general dose-dependent inhibitory effect on protein translation. In the absence of SRP9/14, Alu RNA has a stimulatory effect on all reporter mRNAs. The unstable structure of sRight RNA suggests that the differential activities of Alu RNP and Alu RNA may be explained by conformational changes in the RNA. We demonstrate that Alu RNPs and Alu RNAs do not stably associate with ribosomes during translation and, based on the analysis of polysome profiles and synchronized translation, we show that Alu RNP and Alu RNA regulate translation at the level of initiation.
登录
查看更多内容
影响因子：
14.9
作者：
Bovia, F;Wolff, N;Strub, K
通讯作者：
Strub, K
影响因子：
11.4
作者：
Kreahling, J;Graveley, BR
通讯作者：
Graveley, BR
影响因子：
30.8
作者：
Dewannieux, M;Esnault, C;Heidmann, T
通讯作者：
Heidmann, T
影响因子：
11.4
作者：
Belin, D;Bost, S;Strub, K
通讯作者：
Strub, K
影响因子：
14.9
作者：
Chang, DY;Hsu, K;Maraia, RJ
通讯作者：
Maraia, RJ