Isolation of mycoplasmatales virus-laidlawii 3, a new virus infecting Acholeplasma laidlawii.
Isolation of mycoplasmatales virus-laidlawii 3, a new virus infecting Acholeplasma laidlawii.
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分离支原体病毒-laidlawii 3，一种感染无胆汁原体的新病毒。
DOI：
10.1099/0022-1317-19-2-279
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发表时间：
1973
期刊：
影响因子：
--
通讯作者：
S. Wyld
中科院分区：
文献类型：
--
作者：
R. N. Gourlay;S. Wyld
到目前为止，已经分离出了两种完全不同的感染莱氏无泡球虫的病毒。分别命名为莱氏支原体病毒1(MV-L1)和莱氏支原体病毒2(MV-L2)。MV-L1是一种无包膜的螺旋DNA病毒，直径16 nm，长90 nm(Bruce等人)。1972年)；而MV-L2是一种主要为球形囊膜的病毒，平均直径为80 nm(范围52-125 nm)。它的核酸似乎是DNA(Gourlay等人)。1973年)。
本文报道了另一株感染莱氏艾美耳球虫的病毒的分离，该病毒既不同于MV-L1也不同于MV-L2。
用含5%胎牛血清的磷酸盐缓冲液，将M1305/68株抗MV-L2(Gourlay，1972)莱氏吸虫M1305/68株的两个克隆，在pH 7.3的磷酸盐缓冲液中，连续稀释10倍。将每种稀释液滴入葡萄糖血清(GS)琼脂平板(Gourlay&Wyld，1972)，37℃孵育48h后进行检测。
Two fundamentally different viruses that infect Acholeplasma laidlawii have so far been isolated. They have been designated Mycoplasmatales virus-laidlawii 1 (MV-L1) and Mycoplasmatales virus-laidlawii 2 (MV-L2). MV-L1 is an unenveloped helical DNA virus, 16 nm in diameter and 90 nm in length (Bruce et al. 1972); while MV-L2 is a predominantly spherical enveloped virus with a mean diameter of 80 nm (range 52 to 125 nm). Its nucleic acid appears to be DNA (Gourlay et al. 1973).
This report describes the isolation of another virus which infects A. laidlawii and which differs from both MV-L1 and MV-L2.
Four serial tenfold dilutions of two clones of the M1305/68 strain of Acholeplasma laidlawii resistant to MV-L2 (Gourlay, 1972) were prepared in phosphate-buffered saline, pH 7.3, containing 5% foetal calf serum. Drops (0.02 ml) of each dilution were placed on glucose serum (GS) agar plates (Gourlay & Wyld, 1972) and the plates were examined after incubation at 37 °C for 48 h.