Elderly Patient-Derived Endothelial Cells for Vascularization of Engineered Muscle.
Elderly Patient-Derived Endothelial Cells for Vascularization of Engineered Muscle.
复制标题
老年患者衍生的内皮细胞用于工程肌肉的血管化。
DOI：
10.1016/j.ymthe.2017.02.011
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
S. Levenberg
中科院分区：
文献类型：
--
作者：
Luba Perry;M. Flugelman;S. Levenberg
In vitro prevascularization of engineered tissue constructs promises to enhance their clinical applicability. We hypothesize that adult endothelial cells (ECs), isolated from limb veins of elderly patients, bear the vasculogenic properties required to form vascular networks in vitro that can later integrate with the host vasculature upon implantation. Here, we show that adult ECs formed vessel networks that were more developed and complex than those formed by human umbilical vein endothelial cells (HUVECs) seeded with various supporting cells on three-dimensional (3D) biodegradable polymer scaffolds. In parallel, secreted levels of key proangiogenic cytokines were significantly higher in adult EC-bearing scaffolds as compared to HUVEC scaffolds. As a proof of concept for applicability of this model, adult ECs were co-seeded with human myoblasts as well as supporting cells and successfully formed a branched network, which was surrounded by aligned human myotubes. The vascularized engineered muscle tissue implanted into a full-thickness defect in immunodeficient mice remained viable and anastomosed with the host vasculature within 9 days of implantation. Functional "chimeric" blood vessels and various types of anastomosis were observed. These findings provide strong evidence of the applicability of adult ECs in construction of clinically relevant autologous vascularized tissue.
影响因子：
20.3
作者：
Cheng, Gang;Liao, Shan;Munn, Lance L.
通讯作者：
Munn, Lance L.
影响因子：
15.9
作者：
Felcht, Moritz;Luck, Robert;Augustin, Hellmut G.
通讯作者：
Augustin, Hellmut G.