3-DIMENSIONAL STRUCTURE OF YEAST PHENYLALANINE TRANSFER-RNA - FOLDING OF POLYNUCLEOTIDE CHAIN
3-DIMENSIONAL STRUCTURE OF YEAST PHENYLALANINE TRANSFER-RNA - FOLDING OF POLYNUCLEOTIDE CHAIN
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DOI：
10.1126/science.179.4070.285
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发表时间：
1973-01-01
期刊：
影响因子：
56.9
通讯作者：
RICH, A
中科院分区：
文献类型：
--
作者：
KIM, SH;QUIGLEY, GJ;RICH, A
在 4 Å 分辨率下，可以看到酵母苯丙氨酸转移 RNA 中的多核苷酸呈一系列相距约 5.8 Å 的电子致密质量。这些峰可能与磷酸基团相关，而相邻多核苷酸链片段之间较低水平的电子密度被解释为由氢键结合的嘌呤-嘧啶碱基对产生。仅用两个较小的模糊区域就可以追踪整个多核苷酸链。多核苷酸链具有与三叶草构象一致的二级结构；然而，它的折叠方式与任何模型中提出的折叠方式都不同。该分子由两个相互成直角的L形双链螺旋区域组成。L的一端具有CCA受体；反密码子环位于另一端，二氢尿苷和 TψC 环形成角。
At 4 Å resolution the polynucleotides in yeast phenylalanine transfer RNA are seen in a series of electron dense masses about 5.8 Å apart. These peaks are probably associated with the phosphate groups, while lower levels of electron density between segments of adjacent polynucleotide chains are interpreted as arising from hydrogen-bonded purine-pyrimidine base pairs. It is possible to trace the entire polynucleotide chain with only two minor regions of ambiguity. The polynucleotide chain has a secondary structure consistent with the cloverleaf conformation; however, its folding is different from that proposed in any model. The molecule is made of two double-stranded helical regions oriented at right angles to each other in the shape of anL.One end of theLhas the CCA acceptor; the anticodon loop is at the other end, and the dihydrouridine and TψC loops form the corner.