Ace2p, a regulator of CTS1 (chitinase) expression, affects pseudohyphal production in Saccharomyces cerevisiae
Ace2p, a regulator of CTS1 (chitinase) expression, affects pseudohyphal production in Saccharomyces cerevisiae
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DOI：
10.1007/s002940050384
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发表时间：
1998-09-01
期刊：
影响因子：
2.5
通讯作者：
Butler, G
中科院分区：
文献类型：
--
作者：
King, L;Butler, G
一些酿酒酵母的二倍体菌株可以作为球形酵母形式和丝状假菌丝形式生长。大多数能够形成假菌丝的酵母具有功能性FLO 8基因。我们发现，破坏ACE 2转录因子的结果在生产假菌丝在flo 8 -1的背景。破坏CTS 1（几丁质酶）基因也会在这种背景下产生假菌丝生长，但水平降低。在ACE 2破坏中，单倍体和二倍体细胞对固体培养基的侵袭增加，但二倍体细胞对琼脂的粘附较差。Sigma 1278 b衍生的菌株通常产生假菌丝，其几丁质酶活性比其它菌株低约30倍。
Some diploid strains of Saccharomyces cerevisiae can grow both as a spherical yeast form and as a filamentous pseudohyphal form. Most yeasts capable of forming pseudohyphae possess a functional FLO8 gene. We show that disrupting the ACE2 transcription factor results in the production of pseudohyphae in a flo8-1 background. Disrupting the CTS1 (chitinase) gene also produces pseudohyphal growth in this background, but at a reduced level. Invasion of solid media by haploid and diploid cells is increased in ACE2 disruptions, but the diploids adhere poorly to the agar. Sigma 1278b-derived strains, which generally produce pseudohyphae, have about 30-fold lower chitinase activity than other strains.