MIZ1, an essential protein for root hydrotropism, is associated with the cytoplasmic face of the endoplasmic reticulum membrane in Arabidopsis root cells
MIZ1, an essential protein for root hydrotropism, is associated with the cytoplasmic face of the endoplasmic reticulum membrane in Arabidopsis root cells
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DOI：
10.1016/j.febslet.2012.01.008
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发表时间：
2012-02-17
期刊：
影响因子：
3.5
通讯作者：
Takahashi, Hideyuki
中科院分区：
文献类型：
--
作者：
Yamazaki, Tomokazu;Miyazawa, Yutaka;Takahashi, Hideyuki
MIZ 1是由拟南芥根向水性必需基因编码的。为了表征MIZ 1的性质，我们使用了在miz 1 -1突变体中表达GFP标记的MIZ 1（MIZ 1-GFP）和突变体MIZ 1（MIZ 1（G235 E）-GFP）的转基因植物。尽管两个嵌合基因都被转录，但MIZ 1（G235 E）-GFP的翻译产物在根中不积累。此外，MIZ 1-GFP补充突变表型，但不是MIZ 1（G235 E）-GFP。在皮层细胞和侧根帽细胞中检测到高水平的对应于MIZ 1-GFP的信号，并在皮层细胞的隔室中积累。将MIZ 1-GFP分级分离成可溶性蛋白级分和内质网（ER）膜级分，其中其在胞质侧结合到ER膜的表面。(C)2012年欧洲生物化学学会联合会。由Elsevier B出版。V.保留所有权利。
MIZ1 is encoded by a gene essential for root hydrotropism in Arabidopsis. To characterize the property of MIZ1, we used transgenic plants expressing GFP-tagged MIZ1 (MIZ1-GFP) and mutant MIZ1 (MIZ1(G235E)-GFP) in a miz1-1 mutant. Although both chimeric genes were transcribed, the translational products of MIZ1(G235E)-GFP did not accumulate in roots. Moreover, MIZ1-GFP complemented the mutant phenotype but not MIZ1(G235E)-GFP. The signal corresponding to MIZ1-GFP was detected at high levels in cortical cells and lateral root cap cells and accumulated in compartments in cortical cells. MIZ1-GFP was fractionated into a soluble protein fraction and an endoplasmic reticulum (ER) membrane fraction, where it was bound to the surface of the ER membrane at the cytosolic side. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.