An immunological study on chromogranin A and B in human endocrine and nervous tissues.
An immunological study on chromogranin A and B in human endocrine and nervous tissues.
复制标题
人类内分泌和神经组织中嗜铬粒蛋白 A 和 B 的免疫学研究。
DOI：
10.1007/bf01798500
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Winkler,H
中科院分区：
文献类型：
--
作者：
Schmid,KW;Weiler,R;Xu,RW;Hogue-Angeletti,R;Fischer-Colbrie,R;Winkler,H
根据人嗜铬粒蛋白B的一级氨基酸序列合成的多肽产生抗血清，这些抗血清在一维和二维免疫印迹中识别以前被命名为嗜铬粒蛋白B的成分。在人的嗜铬粒颗粒中，嗜铬粒蛋白B的主要内源加工产物是由靠近C末端的蛋白质的蛋白水解性裂解形成的。用抗人嗜铬颗粒蛋白A和B以及与B序列相对应的合成肽的抗血清进行免疫组织化学定位。在人体组织中，嗜铬粒素B和嗜铬粒素A共存在于肾上腺髓质、垂体前叶、甲状腺滤泡旁细胞、内分泌胰腺的一些细胞和一些肠嗜铬细胞中，而在胃体粘膜的甲状旁腺和肠嗜铬细胞中只发现了嗜铬粒素A。在神经系统中，未见嗜铬颗粒蛋白A的免疫染色，在脊髓的部分细胞中仅见微弱的嗜铬颗粒蛋白B免疫染色。然而，小脑的浦肯野细胞嗜铬粒素B呈强阳性。
Antisera were raised against synthetic peptides derived from the primary amino acid sequence of human chromogranin B. These antisera recognized in one- and two-dimensional immunoblotting a component previously designated as chromogranin B. In human chromaffin granules, the major endogenous processing product of chromogranin B is formed by proteolytic cleavage of the protein near theC-terminus. Immunohistochemical localizations were obtained with antisera against human chromogranins A and B and against a synthetic peptide corresponding to the B sequence. In human tissues, chromogranin B is co-stored with chromogranin A in the adrenal medulla, the anterior pituitary, parafollicular cells of the thyroid, in some cells of the endocrine pancreas and in some enterochromaffin cells, whereas only chromogranin A is found in the parathyroid gland and enterochromaffin cells of the gastric corpus mucosa. In the nervous system, no immunostaining was observed for chromogranin A and only a weak one for chromogranin B in some cells of the spinal cord. However, the Purkinje cells of the cerebellum were strongly positive for chromogranin B.