SMALL ANGLE LIGHT SCATTERING OF BIOLOGICAL CELLS - THEORETICAL CONSIDERATIONS

SMALL ANGLE LIGHT SCATTERING OF BIOLOGICAL CELLS - THEORETICAL CONSIDERATIONS
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DOI:
10.1016/s0006-3495(70)86334-2
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发表时间:
1970-01-01
影响因子:
3.4
通讯作者:
DEAN, PN
DEAN, PN
中科院分区:
生物学3区
文献类型:
--
作者:
MULLANEY, PF;DEAN, PN

文献摘要

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通过霍金森近似和更严格的米氏理论计算了活生物细胞(假设具有相对折射率的均匀球体,m=1.05)以小角度散射的光。两种方法都预测可以根据群体中每个细胞的低角度散射测量来估计相对体积分布。在短波长照射或强吸收的条件下,也可以获得绝对体积信息。
The light scattered by living biological cells (assumed homogeneous spheres with a relative refractive index, m=1.05) at small angles has been calculated by the Hodkinson approximation and the more rigorous Mie theory. Both methods predict that relative volume distributions may be estimated from low angle scattering measurements on each cell in a population. Under conditions of short wavelength illumination or strong absorption, absolute volume information may also be obtained.