FLUORESCENCE IN ARTHROPODA INFORMS ECOLOGICAL STUDIES IN ANCHIALINE CRUSTACEANS, REMIPEDIA, AND ATYIDAE
FLUORESCENCE IN ARTHROPODA INFORMS ECOLOGICAL STUDIES IN ANCHIALINE CRUSTACEANS, REMIPEDIA, AND ATYIDAE
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DOI：
10.1163/1937240x-00002170
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发表时间：
2013-09-01
影响因子：
1.1
通讯作者：
Caldwell, Roy L.
中科院分区：
文献类型：
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作者：
Glenn, Dawn;Pakes, M. Joey;Caldwell, Roy L.
自体荧光普遍存在于自然界，存在于许多动物、植物和矿物中。在节肢动物外骨骼的蜕皮周期中，荧光特性的变化是可以预测的。这项研究使用新的荧光分析和显微镜检查了蝎子和甲壳类动物的本征荧光。我们在这里报道的安氏洞穴甲壳类动物，通常种群稀少，研究人员很难接近。因此，人们对其特有居民的生长、蜕皮、繁殖和生活史知之甚少。本文提出的在近紫外光波长下测量自体荧光的方法对于最佳的原位观察和定位新的幼虫形态、蜕皮、精原和卵是有价值的。我们比较了加州林地蝎子(Uroctonus Mordax)和加州海湾对虾(Crangon Franciscorum)提取的荧光化合物的强度和发射宽度，以确定保存时间对自体荧光性质的影响。从墨西哥Quintana Roo的Cenote甲壳类和巴哈马的Abaco岛的Dan‘s Cave采集到的Anchialine洞穴甲壳动物的荧光数据。它们的峰值发射波长从429 nm到515 nm不等，发射峰存在种间差异。与发射光谱有关的特征，如广度和强度，可能与各种生态参数相关，如性别、年龄和生殖状态，从而允许潜在的现场和实验室应用。
Autofluorescence is pervasive throughout the natural world and occurs in many animals, plants, and minerals. Fluorescent properties vary predictably over molt cycles of arthropod exoskeletons. This study examines intrinsic fluorescence in scorpions and crustaceans using novel fluorometric analyses and microscopy. Anchialine cave crustaceans, which we report here autofluoresce, are generally sparsely populated, and difficult for researchers to access. Therefore, little is known about the growth, molting, reproduction, and life history of their endemic inhabitants. Methods proposed here for measuring autofluorescence under near-ultraviolet wavelengths are valuable in informing techniques for optimal in situ observation and location of new larval forms, exuviae, spermataphores and eggs. We compare the intensity and emission breadth of fluorescent compounds extracted from California woodland scorpions (Uroctonus mordax) and California bay shrimp (Crangon franciscorum) to determine the effect of preservation duration on autofluorescent properties. Fluorescence data were obtained from anchialine cave crustaceans collected from Cenote Crustacea, Quintana Roo, Mexico and Dan's Cave, Abaco Island, Bahamas. They exhibit peak emission wavelengths ranging from 429 nm to 515 nm and interspecific variation in emission peaks. Traits relating to emission spectra, such as breadth and intensity, may correlate with a variety of ecological parameters, such as sex, age, and reproductive state, allowing for potential field and laboratory-based applications.