The effect of IL-1β on the expression of inflammatory cytokines and their receptors in human chondrocytes
The effect of IL-1β on the expression of inflammatory cytokines and their receptors in human chondrocytes
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DOI：
10.1016/j.lfs.2006.02.038
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发表时间：
2006-07-17
期刊：
影响因子：
6.1
通讯作者：
Makimura, Masaharu
中科院分区：
文献类型：
--
作者：
Aida, Yukiko;Maeno, Masao;Makimura, Masaharu
在炎症和感染部位释放的细胞因子可以改变软骨转换的正常过程，导致病理性破坏或形成。白细胞介素(IL)-1 β在关节炎软骨损伤和退化的病理生理中起核心作用。在本研究中，我们检测了IL-1 β对人软骨细胞中IL-1 β、IL-6、IL-8、IL-11、肿瘤坏死因子-a （tnf - α）及其受体表达的影响。细胞在添加或不添加100 U/ml IL-1 β的情况下培养28天。通过实时PCR测定mRNA水平或ELISA测定蛋白水平来估计细胞因子及其受体的表达水平。培养第14天，IL-1 β、IL-8和tnf - α的表达在IL-1 β存在下显著升高。在IL-1 β存在的情况下，IL-6和IL-11的表达在培养第1天和第14天后显著增加。培养第14天，在IL-10的存在下，IL-1 β、IL-8、IL-11和tnf - α受体的表达显著降低，IL-6受体的表达显著升高。除IL-6外，这些细胞因子的表达随着人IL-1受体拮抗剂的加入而降低。这些结果表明，IL-1 β通过增加软骨细胞产生炎性细胞因子来促进软骨基质周转的分解系统，并可能通过增加细胞中IL-6受体的表达来促进IL-6的自分泌作用。(c) 2006爱思唯尔公司版权所有。
Cytokines released at sites of inflammation and infection can alter the normal processes of cartilage turnover, resulting in pathologic destruction or formation. Interleukin (IL)-1 beta plays a central role in the pathophysiology of cartilage damage and degradation in arthritis. In the present study, we examined the effect of IL-1 beta on the expression of IL-1 beta, IL-6, IL-8, IL-11, tumor necrosis factor-a (TNF-alpha), and their receptors in human chondrocytes. The cells were cultured either with or without 100 U/ml of IL-1 beta for up to 28 days. The level of expression of the cytokines and their receptors was estimated by determining mRNA levels using real-time PCR or by determining protein levels using ELISA. The expression of IL-1 beta, IL-8, and TNF-alpha markedly increased in the presence of IL-1 beta after day 14 of culture. The expression of IL-6 and IL-11 increased greatly in the presence of IL-1 beta on day I and after day 14 of culture. The expression of IL-1 beta, IL-8, IL-11, and TNF-alpha receptors significantly decreased in the presence of IL-10 after day 14 of culture, whereas the expression of IL-6 receptor significantly increased. The expression of these cytokines, except for IL-6, decreased with the addition of human IL-1 receptor antagonist. These results suggest that IL-1 beta promotes the resolution system of cartilage matrix turnover through an increase in inflammatory cytokine production by chondrocytes and that it also may promote the autocrine action of IL-6 through an increase in IL-6 receptor expression in the cells. (c) 2006 Elsevier Inc. All rights reserved.