Spontaneously Occurring Hepatocellular Neoplasia in Adolescent Cynomolgus Monkeys (Macaca fascicularis)
Spontaneously Occurring Hepatocellular Neoplasia in Adolescent Cynomolgus Monkeys (Macaca fascicularis)
复制标题
青少年食蟹猴（食蟹猴）自发发生的肝细胞肿瘤
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
W. Bobrowski
中科院分区：
文献类型：
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作者：
J. Reindel;K. Walsh;K. Toy;W. Bobrowski
在两只青春期（<5 岁）雄性食蟹猴（Macaca fasciculis）中发现自发性肝肿瘤。 1 号猴子患有孤立性肝细胞癌 (HCC)。 2 号猴子患有多个离散肿瘤，其中包括几个边界不清的 HCC 和一个混合性肝胆管细胞癌 (MHC)。两只猴子的转移均不明显。使用组织化学和免疫组织化学染色来评估肿瘤的表型改变。两只猴子的许多或大多数肿瘤性肝细胞 (NH) 呈低分子量细胞角蛋白 (LMWCK)、细胞角蛋白 (CK) 8 和 CK 18 染色阳性。在 1 号猴中，小团 NH 呈癌胚抗原 (CEA)、谷胱甘肽 S-转移酶-pi (GST) 和甲胎蛋白 (AFP) 阳性，但 NH 呈阳性。 CK 7 一致阴性。2 号猴的 NH 的 CEA 和 AFP 阴性，但 GST 和 CK 7 多灶性阳性。广谱细胞角蛋白 (BSCK)、高分子量细胞角蛋白 (HMWCK) 和 CK 19 不与任一动物的 NH 发生反应。 2 号猴的 MHC 中形成导管样结构的肿瘤细胞用 LMWCK、CK 7、CK8、CK 18、BSCK 和 GST 染色，但不用 HMWCK 或 CK 19 染色。两只猴子的肿瘤细胞周围纤连蛋白染色均增强。两只猴子的非肿瘤性实质均含有多个离散的细胞改变灶和分散的肝细胞聚集体，具有强纤连蛋白细胞质染色。这些肿瘤的染色模式证明了个体肿瘤内肿瘤细胞的免疫表型异质性以及肿瘤之间的变异性。该信息可以为其他在灵长类动物中遇到类似病变的人提供有用的参考。
Spontaneous hepatic neoplasms were identified in two adolescent (<5 years of age) male cynomolgus monkeys (Macaca fascicularis). Monkey No. 1 had a solitary hepatocellular carcinoma (HCC). Monkey No. 2 had multiple discrete tumors consisting of several poorly circumscribed HCCs and a mixed hepatocholangiocellular carcinoma (MHC). Metastases were not evident in either monkey. Histochemical and immunohistochemical stains were used to assess phenotypic alterations in the tumors. Many or most neoplastic hepatocytes (NHs) of both monkeys stained positive for low-molecular-weight cytokeratin (LMWCK), cytokeratin (CK) 8, and CK 18. In monkey No. 1, small aggregates of NHs were positive for carcinoembryonic antigen (CEA), glutathione S-transferase-pi (GST), and α-fetoprotein (AFP), but NHs were uniformly negative for CK 7. NHs in monkey No. 2 were negative for CEA and AFP but were multifocally positive for GST and CK 7. Broad-spectrum cytokeratin (BSCK), high-molecular-weight cytokeratin (HMWCK), and CK 19 did not react with NHs of either animal. Neoplastic cells forming ductlike structures in the MHC of monkey No. 2 stained with LMWCK, CK 7, CK8, CK 18, BSCK, and GST but not with HMWCK or CK 19. Tumors in both monkeys had enhanced pericellular fibronectin staining. Nonneoplastic parenchyma of both monkeys contained multiple discrete foci of cellular alteration and scattered aggregates of hepatocytes with strong cytoplasmic staining for fibronectin. Staining patterns of these tumors demonstrate immunophenotypic heterogeneity of the neoplastic cells within individual tumors and variability among tumors. This information may serve as a useful reference for others encountering similar lesions in primates.
人类肝脏再生的形态学和免疫组织化学特征。
DOI：
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发表时间：
1995
期刊：
The American journal of pathology.
影响因子：
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作者：
Rubin,EM;Martin,AA;Thung,SN;Gerber,MA
通讯作者：
Gerber,MA