Control of precerebellar neuron development by Olig3 bHLH transcription factor.
Control of precerebellar neuron development by Olig3 bHLH transcription factor.
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DOI：
10.1523/jneurosci.3769-08.2008
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发表时间：
2008-10-01
期刊：
影响因子：
--
通讯作者：
Qiu M
中科院分区：
文献类型：
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作者：
Liu Z;Li H;Hu X;Yu L;Liu H;Han R;Colella R;Mower GD;Chen Y;Qiu M
菱形唇(RL)是紧邻第四脑室顶板的神经上皮细胞，可形成多种脑干和小脑细胞。我们的研究表明，碱性螺旋-环-螺旋(BHLH)转录因子Orig3在RL的祖细胞中表达，并且Opol3的去掉对RL的发育有显著影响。在寡核苷酸−/−尾侧RL，Math1在DI1区的表达水平降低，四个苔藓纤维核的形成受到影响，DI2-DI3神经元被错误地指定为DI4中间神经元，攀爬纤维神经元(下橄榄核)完全消失。此外，脑干(NOR)肾上腺素能中枢和一阶中继性内脏感觉神经元的形成也依赖于寡核苷酸。因此，在尾侧RL来源神经元的命运指定和分化过程中，Orig3起着重要的作用。
The rhombic lip (RL) is the neuroepithelium immediately adjacent to the roof plate of the fourth ventricle and it gives rise to various brainstem and cerebellar cell types. Our study shows that the basic helix-loop-helix (bHLH) transcription factor Olig3 is expressed in the progenitors of RL and ablation of Olig3 significantly affects the development of RL. In Olig3−/− caudal RL, the expression level of Math1 in the dI1 domain is reduced and the formation of four mossy-fiber nuclei is compromised; dI2–dI3 neurons are mis-specified to dI4 interneurons and the climbing-fiber neurons (inferior olive nucleus) are completely lost. In addition, the formation of brainstem (nor)adrenergic centers and first-order relay visceral sensory neurons is also dependent on Olig3. Therefore, Olig3 plays an important role in the fate specification and differentiation of caudal RL-derived neurons.