Characterization of tunable FGF-2 releasing polyelectrolyte multilayers.
Characterization of tunable FGF-2 releasing polyelectrolyte multilayers.
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DOI：
10.1021/bm100413w
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发表时间：
2010-08-09
影响因子：
6.2
通讯作者：
Hammond, Paula T.
中科院分区：
文献类型：
--
作者：
Macdonald, Mara L.;Rodriguez, Natalia M.;Shah, Nisarg J.;Hammond, Paula T.
成纤维细胞生长因子2 （FGF-2）是干细胞分化和增殖的有效介质。尽管FGF-2在促进骨组织形成方面具有良好的作用，但其递送缺陷限制了其临床应用。聚电解质多层膜代表了一种新的FGF-2递送系统，有望从感兴趣的表面（包括医疗植入物和组织工程支架）局部、精确控制和持续释放FGF-2。在这项工作中，研究了不同结构的合成水解可降解多层薄膜中FGF-2的加载和释放；通过至少三个参数（纳米层数、反聚阴离子和可降解聚阳离子类型）可调节载药量，FGF-2的产率为7-45 ng/cm2。释放时间从24小时到大约5天不等。从这些膜中释放的FGF-2保留了体外活性，促进了MC3T3前成骨细胞的增殖。在多层结构中使用生物来源的反聚阴离子硫酸肝素和硫酸软骨素增强了FGF-2的活性。对药物装载和释放动力学的控制为未来的体内骨和组织再生模型提供信息，以探索LbL生长因子递送膜的临床相关性。
Fibroblast growth factor 2 (FGF-2) is a potent mediator of stem cell differentiation and proliferation. Although FGF-2 has a well-established role in promoting bone tissue formation, flaws in its delivery have limited its clinical utility. Polyelectrolyte multilayer films represent a novel system for FGF-2 delivery that has promise for local, precisely controlled and sustained release of FGF-2 from surfaces of interest including medical implants and tissue engineering scaffolds. In this work, the loading and release of FGF-2 from synthetic hydrolytically degradable multilayer thin films of various architectures is explored; drug loading was tunable using at least three parameters (number of nanolayers, counterpolyanion, and type of degradable polycation) and yielded values of 7-45 ng/cm2 of FGF-2. Release time varied between 24 hours and approximately five days. FGF-2 released from these films retained in vitro activity, promoting the proliferation of MC3T3 pre-osteoblast cells. The use of biologically derived counterpolyanions heparin sulfate and chondroitin sulfate in the multilayer structures enhanced FGF-2 activity. The control over drug loading and release kinetics inform future in vivo bone and tissue regeneration models for the exploration of clinical relevance of LbL growth factor delivery films.
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影响因子：
3.3
作者：
Bonora-Centelles, A.;Jover, R.;Gomez-Lechon, M. J.
通讯作者：
Gomez-Lechon, M. J.
影响因子：
5.5
作者：
Lavalle, P;Gergely, C;Picart, C
通讯作者：
Picart, C
DOI：
10.1016/j.bbrc.2010.03.174
发表时间：
2010-05-07
影响因子：
3.1
作者：
Kang, Kyungjun;Song, Mi-Ryoung
通讯作者：
Song, Mi-Ryoung
影响因子：
6.2
作者：
Khopade, AJ;Arulsudar, N;Hartmann, J
通讯作者：
Hartmann, J
MAD（多药剂输送）纳米层：通过分层组装的表面涂层输送多种治疗药物。
DOI：
10.1021/la9017618
发表时间：
2009-12-15
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Kim BS;Smith RC;Poon Z;Hammond PT
通讯作者：
Hammond PT