Selecting Female Mice in Estrus and Checking Plugs.
Selecting Female Mice in Estrus and Checking Plugs.
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DOI：
10.1101/pdb.prot092387
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发表时间：
2016-08-01
影响因子：
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通讯作者：
Nagy, Andras
中科院分区：
文献类型：
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作者：
Behringer, Richard;Gertsenstein, Marina;Nagy, Andras
雌性小鼠的发情周期分为四个阶段：发情前期（卵泡发育）、发情期（排卵）、发情后期（黄体形成）和发情间期（卵泡开始发育以进行下一次排卵并消除先前的卵母细胞）。外生殖器上皮的外观用于识别雌性小鼠动情周期的阶段。这通常在没有或仅有浅色皮肤色素沉着的菌株中更容易看到。通过检查阴道的颜色、湿润度和肿胀程度，可以很容易地识别出处于发情期的雌性。为了进行交配，下午对雌性进行检查，并将发情期的雌性与雄性一起放入笼子中（每个笼子里有一只或两只雌性，一只雄性）。通常，50%或更多的选定雌性会交配。第二天早上出现阴道交配塞表明交配已经发生，但这并不意味着即使使用经过证明的可繁育的可育雄性也会怀孕。清晨检查阴道塞非常重要，因为它们会在交配后约 12 小时或更早时脱落或不再被发现。
The female mouse estrous cycle is divided into four phases: proestrus (development of ovarian follicles), estrus (ovulation), metestrus (formation of corpora lutea), and diestrus (beginning of follicle development for next ovulation and elimination of previous oocytes). The appearance of the epithelium of the external genitalia is used to identify the stage of the estrous cycle of a female mouse. This is usually easier to see in strains with either no or only light skin pigmentation. By examining the color, moistness, and degree of swelling of the vagina, females in estrus can readily be identified. To set up the matings, females are examined in the afternoon, and those in estrus are placed into the cages with males (one or two females in each cage with one male). Usually, 50% or more of the selected females will mate. The presence of a vaginal copulation plug next morning indicates that mating has occurred, but it does not mean that a pregnancy will result even if proven breeder fertile males were used. It is important to check vaginal plugs early in the morning because they fall out or are no longer detectable ~12 h after mating or sometimes earlier.