Estrogen induced expression of the C-jun proto-oncogene in the immature and mature rat uterus.
Estrogen induced expression of the C-jun proto-oncogene in the immature and mature rat uterus.
复制标题
雌激素诱导未成熟和成熟大鼠子宫中 C-jun 原癌基因的表达。
DOI：
10.1016/0006-291x(90)92381-9
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发表时间：
1990
影响因子：
3.1
通讯作者：
Khan,SA
中科院分区：
文献类型：
--
作者：
Webb,DK;Moulton,BC;Khan,SA
使用编码小鼠c-jun原癌基因的cDNA测量未成熟和成熟大鼠子宫组织中对雌二醇处理的原癌基因c-jun的表达。该探针与2.7kb的主要RNA条带和3.2kb的次要RNA条带杂交。在来自未成熟和成熟大鼠子宫的总RNA的北方印迹中，注射后3小时，雌二醇处理导致2.7 kb条带的表达比对照水平至少增加3倍。注射后12小时，c-junmRNA的表达已恢复到对照水平。在用雌二醇处理后约3小时，在从成熟大鼠分离的基质-子宫肌层组织中也观察到c-junmRNA表达的强烈诱导（>5倍）。c-fos和c-jun诱导的相似动力学强调了fos/jun异源二聚体在控制子宫细胞增殖中的功能意义。
The expression of the proto-oncogene c-junin response to estradiol treatment in immature and mature rat uterine tissue was measured using a cDNA encoding the mouse c-junproto-oncogene. This probe hybridized to a major RNA band of 2.7 kb and a minor 3.2 kb band. In Northern blots of total RNA from both immature and mature rat uteri, estradiol treatment resulted in at least a 3 fold increase in expression of the 2.7 kb band over control levels by 3 hr post injection. By 12 hr post injection, expression of c-junmRNA had returned to control levels. A strong induction (>5 fold) of c-junmRNA expression was also observed in stroma-myometrial tissue isolated from mature rats approximately 3 hours after treatment with estradiol. The similar kinetics of induction of c-fosand c-junemphasizes the functional significance of thefos/junheterodimer in control of uterine cell proliferation.