COEXPRESSION OF KERATIN-TYPE AND VIMENTIN-TYPE INTERMEDIATE FILAMENTS IN HUMAN METASTATIC CARCINOMA-CELLS
COEXPRESSION OF KERATIN-TYPE AND VIMENTIN-TYPE INTERMEDIATE FILAMENTS IN HUMAN METASTATIC CARCINOMA-CELLS
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DOI：
10.1073/pnas.80.9.2618
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
VOOIJS, GP
中科院分区：
文献类型：
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作者：
RAMAEKERS, FCS;HAAG, D;VOOIJS, GP
通过在间接免疫荧光技术中使用角蛋白、波形蛋白和结蛋白的抗体，检查存在于人类浆液腔液体（腹水或胸水）的积液中的上皮来源的转移性肿瘤细胞的中等大小的丝状类型。实体上皮肿瘤（原发癌及其转移瘤）只含有中等大小的角蛋白丝。然而，当这些细胞存在于腹水或胸膜液中时，它们也表达波形蛋白，波形蛋白出现在纤维组织中。讨论了这种额外但暂时的细胞骨架对转移性生长或侵袭性（或两者）的可能影响。
Metastatic tumor cells of epithelial origin present in effusions from human serous cavity fluids (ascites or pleural fluid) were examined for their intermediate-sized filament types by using antibodies to keratin, vimentin, and desmin in the indirect immunofluorescence technique. Solid epithelial tumors (both primary carcinomas and their metastases) contain keratin intermediate-sized filaments exclusively. However, when these cells are present in ascitic or pleural fluid, they also express vimentin, which occurs in a fibrillar organization. The possible effects of this additional, but temporary, cytoskeleton on metastatic growth or aggressiveness (or both) are discussed.