Amphiphilic and biodegradable methoxy polyethylene glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl methacrylate)) as an effective gene carrier.
Amphiphilic and biodegradable methoxy polyethylene glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl methacrylate)) as an effective gene carrier.
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DOI：
10.1016/j.biomaterials.2010.09.052
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发表时间：
2011-01
期刊：
影响因子：
14
通讯作者：
Liang, Xing-Jie
中科院分区：
文献类型：
--
作者：
Guo, Shutao;Huang, Yuanyu;Wei, Tuo;Zhang, Wendi;Wang, Weiwei;Lin, Daoshu;Zhang, Xu;Kumar, Anil;Du, Quan;Xing, Jinfeng;Deng, Liandong;Liang, Zicai;Wang, Paul C.;Dong, Anjie;Liang, Xing-Jie
关键词：
采用开环聚合和原子转移自由基聚合相结合的方法合成了一组两亲性阳离子聚合物甲氧基聚乙烯(glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl)。用核磁共振氢谱对两亲阳离子聚合物的结构进行了表征。PECD纳米粒子具有覆盖有亲水性的聚乙二醇链和阳离子PDMAEMA链的疏水核心。用动态光散射(DLS)技术对这些自组装纳米粒子进行了表征。PECD纳米粒子可以有效地凝聚DNA，形成尺寸为65-160 nm的致密复合体，适合于基因传递。体外基因转染HeLa和HepG2细胞的研究表明，在低剂量(N/P=5)下，PECD纳米粒的转染率高于聚乙烯亚胺(PEI)和脂质体2000(N/P=5)。细胞毒性实验结果表明，在最佳N/P比条件下制备的PECD纳米粒/DNA复合体具有与PEI和脂质体相当的细胞毒性。这些结果表明，PECD纳米颗粒有很大的潜力作为高效的聚合物载体用于基因转染。
A group of amphiphilic cationic polymers, methoxy polyethylene glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl methacrylate)) (PECD), were synthesized by combining ring-opening polymerization (ROP) and atom transfer radical polymerization (ATRP) methods to form nanoparticles (NPs). The structures of these amphiphilic cationic polymers were characterized by 1H NMR measurement. The PECD NPs have hydrophobic cores covered with hydrophilic PEG and cationic PDMAEMA chains. These self-assembly nanoparticles were characterized by dynamic light scattering (DLS) technique. PECD NPs can effectively condense DNA to form compact complexes of the size 65–160 nm suitable for gene delivery. The in vitro gene transfection studies of HeLa and HepG2 cells show that PECD NPs have better transfection efficiency compared to polyethylenimine (PEI) and Lipofectamine 2000 at low dose (N/P = 5). The cytotoxicity result shows that PECD NPs/DNA complexes at the optimal N/P ratio for transfection have comparable toxicity with PEI and Lipofectamine. These results indicate that PECD NPs have a great potential to be used as efficient polymeric carriers for gene transfection.
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影响因子：
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