Vitreous Humor Changes Expression of Iron-Handling Proteins in Lens Epithelial Cells.
Vitreous Humor Changes Expression of Iron-Handling Proteins in Lens Epithelial Cells.
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DOI：
10.1167/iovs.16-20610
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发表时间：
2017-02-01
影响因子：
4.4
通讯作者：
McGahan MC
中科院分区：
文献类型：
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作者：
Goralska M;Fleisher LN;McGahan MC
在人类中，玻璃体切除术与核性白内障的发展相关。铁催化自由基形成，引起氧化损伤，这与白内障形成有关。这项研究旨在确定玻璃体液，它可以启动透镜上皮细胞的分化，是否会对铁处理蛋白产生影响。用收集的犬玻璃体液处理培养的犬透镜上皮细胞。通过SDS-PAGE分离处理细胞和对照细胞的裂解物。铁蛋白H-和L-链，转铁蛋白受体1，和水通道蛋白0进行免疫检测和定量与特定的抗体。评估处理细胞的形态学变化。用33%（体积/体积）的玻璃体液溶液处理透镜上皮细胞改变了透镜细胞的形态，并诱导水通道蛋白0的表达，水通道蛋白0是纤维细胞分化的标志物，在对照细胞中检测不到。治疗没有改变铁处理蛋白的大小，但显着增加铁蛋白的含量从2.9到8.8倍，超过对照组和转铁蛋白受体水平下降了37%至59%。玻璃体液可显著限制转铁蛋白/转铁蛋白受体途径的铁摄取，并通过增加铁蛋白水平可显著增加透镜细胞中铁蛋白的铁储存能力。玻璃体液可能对透镜上皮细胞的铁催化氧化损伤起重要的保护作用，因此在白内障的形成中起重要作用。
In humans, vitrectomy is associated with development of nuclear cataracts. Iron catalyzes free radical formation causing oxidative damage, which is implicated in cataract formation. This study was designed to determine if vitreous humor, which can initiate differentiation of lens epithelial cells, would have an effect on iron-handling proteins. Cultured canine lens epithelial cells were treated with collected canine vitreous humor. Lysates of treated and control cells were separated by SDS-PAGE. Ferritin H- and L-chains, transferrin receptor 1, and aquaporin 0 were immunodetected and quantitated with specific antibodies. Morphologic changes in treated cells were assessed. Treatment of lens epithelial cells with a 33% (vol/vol) solution of vitreous humor changed the morphology of lens cells and induced expression of aquaporin 0, a marker of fiber cell differentiation that was undetectable in control cells. Treatment did not modify the size of iron-handling proteins but significantly increased content of ferritin from 2.9- to 8.8-fold over control and decreased levels of transferrin receptor by 37% to 59%. Vitreous humor may significantly limit iron uptake by transferrin/transferrin receptor pathway, and by increasing ferritin levels could profoundly increase the iron-storage capacity of ferritin in lens cells. Vitreous humor may play a significant protective role against iron-catalyzed oxidative damage of lens epithelial cells and therefore in the formation of cataracts.