beta 2-Glycoprotein I: a plasma inhibitor of the contact activation of the intrinsic blood coagulation pathway.
beta 2-Glycoprotein I: a plasma inhibitor of the contact activation of the intrinsic blood coagulation pathway.
复制标题
β 2-糖蛋白 I：内在凝血途径接触激活的血浆抑制剂。
作者：
I. Schousboe
β 2-糖蛋白 I 的生物学功能的一般假设是，它可以中和所有可能进入血液的带负电荷的大分子，并减少不必要的凝血激活。在本研究中，我们报告 β2-糖蛋白 I 抑制凝血内在途径的接触相系统的激活。通过鞣花酸-磷脂悬浮液 (Cephotest) 完成激活，并使用显色底物 H-D-Pro-Phe-Arg-p-硝基苯胺 (S-2302) 通过酰胺分解活性的出现来测量。当 Cephotest 与 β 2-糖蛋白 I 预孵育时，以及通过向正常或 β 2-糖蛋白 I 缺乏的血浆中添加 β 2-糖蛋白 I 来增加血浆中 β 2-糖蛋白 I 的量时，均观察到 β 2-糖蛋白 I 的这种抑制作用。 β2-糖蛋白I对接触相激活的抑制作用可能是该蛋白的生理功能之一。
The general hypothesis for the biological function of beta 2-glycoprotein I is that it neutralizes all negatively charged macromolecules that might enter the bloodstream and diminishes unwanted activation of the blood coagulation. In the present study we report that beta 2-glycoprotein I inhibits the activation of the contact phase system of the intrinsic pathway of blood coagulation. Activation was accomplished by an ellagic acid-phospholipid suspension (Cephotest) and measured by the appearance of amidolytic activity using the chromogenic substrate H-D-Pro-Phe-Arg-p-nitroanilide (S-2302). This inhibitory effect of beta 2-glycoprotein I was observed both when Cephotest was preincubated with beta 2-glycoprotein I and when the amount of beta 2-glycoprotein I in plasma was increased by addition of beta 2-glycoprotein I to either normal or beta 2-glycoprotein I-deficient plasma. The inhibitory effect of beta 2-glycoprotein I on the contact phase activation could be one of the physiological functions of this protein.
复合因子 V/VIII 缺乏症中活化蛋白 C 抑制剂功能正常水平的证据。
DOI：
10.1172/jci110725
发表时间：
1982
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Canfield,WM;Kisiel,W
通讯作者：
Kisiel,W