Evaluation of pulsed-field gel electrophoresis for typing of Shigella dysenteriae type 1.
Evaluation of pulsed-field gel electrophoresis for typing of Shigella dysenteriae type 1.
复制标题
脉冲场凝胶电泳对 1 型痢疾志贺菌分型的评价。
DOI：
10.1099/00222615-48-8-781
复制
发表时间：
1999
影响因子：
3
通讯作者：
M. Albert
中科院分区：
文献类型：
--
作者：
K. Talukder;Dilip K. Dutta;M. Albert
脉冲场凝胶电泳(PFGE)已被成功地用于区分许多不同细菌种类的菌株。本研究用限制性内切酶NotI和PFGE消化细菌DNA，对流行痢疾的重要病原--1型志贺氏菌进行分型。从4次痢疾暴发中分离到27株，从地方性痢疾病例和实验室培养标本中分离到44株。流行株分为两个类型，各有两个亚型，而地方性分离株和培养物有8个类型，有多个亚型。这些发现表明，沙门氏菌1型可以用PFGE进行分型。
Pulsed-field gel electrophoresis (PFGE) has been used successfully to discriminate between strains of many different bacterial species. In this study, digestion of bacterial DNA with the restriction endonuclease NotI and PFGE were evaluated for the typing of isolates of Shigella dysenteriae type 1, an important cause of epidemic dysentery. There were 27 isolates from four outbreaks of dysentery, and 44 isolates from endemic dysentery cases and a laboratory culture collection. The epidemic isolates yielded two types each with two subtypes, whereas the endemic isolates and culture collection yielded eight types with numerous subtypes. These findings suggest that S. dysenteriae 1 can be typed by PFGE.