Irreversible regional cerebral ischemia: serial MR imaging and proton MR spectroscopy in a nonhuman primate model.
Irreversible regional cerebral ischemia: serial MR imaging and proton MR spectroscopy in a nonhuman primate model.
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发表时间：
1993-07
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通讯作者：
L. Monsein;V. Mathews;P. Barker;C. Pardo;S. Blackband;W. D. Whitlow;D. Wong;R. Bryan
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作者：
L. Monsein;V. Mathews;P. Barker;C. Pardo;S. Blackband;W. D. Whitlow;D. Wong;R. Bryan
描述狒狒基底神经节急性不可逆缺血时质子磁共振波谱和成像的变化。材料与方法：采用微导管和N-丁基氰基丙烯酸酯粘合剂，通过血管内方法闭塞6只成年雄性狒狒的大脑中动脉M1段。脑血流量测量正电子发射断层扫描或放射性微球技术。对基底节区进行了连续的空间定位质子磁共振波谱分析，并对脑进行了磁共振成像。缺血和梗死组织的分布通过组织病理学技术或氯化三苯基四氮唑染色来证明。结果放射性微球或正电子发射断层扫描测量显示大脑中动脉闭塞后基底节内无明显脑血流。基底神经节的质子磁共振波谱显示大脑中动脉闭塞30分钟内大脑乳酸增加和N-乙酰天冬氨酸减少。基底节区的MR成像信号强度变化在T2加权成像上最早观察到3.1小时，在T1加权成像上观察到3.3小时，在自旋密度加权成像上观察到6.1小时。这些变化的分布与整个基底节的缺血和梗死的组织病理学特征相关。结论在我们的狒狒基底节急性不可逆缺血模型中，MRI信号强度的变化与缺血和梗死相对应。脑乳酸增加和N-乙酰天冬氨酸减少先于MR成像信号强度的变化。
PURPOSE To delineate the changes in proton MR spectroscopy and imaging that occur with acute, irreversible ischemia of the basal ganglia of a baboon. MATERIALS AND METHODS The M1 segments of the middle cerebral arteries of six adult male baboons were occluded by endovascular means with microcatheters and N-butyl cyanoacrylate adhesive. Cerebral blood flow measurements were taken with positron emission tomography or radioactive microsphere techniques. Serial spatially localized proton MR spectroscopy of the basal ganglia and MR imaging of the brain were performed. The distribution of ischemic and infarcted tissue was demonstrated by histopathologic techniques or triphenyltetrazolium chloride staining. RESULTS Radioactive microsphere or positron emission tomography measurements demonstrated no significant cerebral blood flow within the basal ganglia after occlusion of the middle cerebral artery. Proton MR spectroscopy of the basal ganglia demonstrated increasing cerebral lactate and decreasing N-acetyl aspartate within 30 minutes of middle cerebral artery occlusion. Changes in the MR imaging signal intensity of the basal ganglia were observed as early as 3.1 hours on T2-weighted, 3.3 hours on T1-weighted, and 6.1 hours on spin density-weighted images. The distribution of these changes correlated well with the histopathologic features of ischemia and infarction that were seen throughout the basal ganglia. CONCLUSION Changes in MR imaging signal intensity corresponded to ischemia and infarction in our baboon model of acute irreversible ischemia of the basal ganglia. Increasing cerebral lactate and decreasing N-acetyl aspartate preceded changes in MR imaging signal intensity.