Inner ear proteomics: a fad or hear to stay.
Inner ear proteomics: a fad or hear to stay.
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内耳蛋白质组学：时尚或留存。
DOI：
10.1016/j.brainres.2006.01.099
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Thalmann,Isolde
中科院分区：
文献类型：
--
作者：
Thalmann,Isolde
蛋白质组学是对蛋白质的结构和功能以及蛋白质-蛋白质相互作用的大规模分析，已经发展成为“系统分析”的主要组成部分。这需要整合来自不同来源和多个水平的信息，并涉及两个不同的参数，（1）高通量蛋白质分离，鉴定和表征，以及（2）扩展所获得的分析数据以确定生理功能。内耳由于其微小的体积、较差的可及性、与复杂的流体空间的关联以及细胞类型的多样性，对蛋白质组学的研究提出了特殊的挑战。介绍了内耳蛋白质组学研究的各种方法，并讨论了成功的故事，值得注意的失败和未来的发展。
Proteomics, the large-scale analysis of the structure and function of proteins, as well as of protein–protein interactions, has evolved into a major component of ‘systems analysis’. This requires the integration of information from different sources and at multiple levels, and involves two distinct parameters, (1) high-throughput protein separation, identification, and characterization, and (2) the extension of the obtained analytical data for the determination of the physiological function. The inner ear poses exceptional challenges to the study of proteomics because of its minute size, poor accessibility, association with complex fluid spaces, and diversity of cell types. Various approaches to the study of proteomics of the inner ear are presented, and success stories, noteworthy failures and what lies ahead, will be discussed.