A20, an inhibitor of cell death, self-associates by its zinc finger domain
A20, an inhibitor of cell death, self-associates by its zinc finger domain
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DOI：
10.1016/0014-5793(96)00283-9
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发表时间：
1996-04-08
期刊：
影响因子：
3.5
通讯作者：
Fiers, W
中科院分区：
文献类型：
--
作者：
DeValck, D;Heyninck, K;Fiers, W
A20 是单核细胞粘附或多种细胞的细胞因子刺激后诱导的初级反应基因，A20 蛋白属于一类新型 Cys(2)/Cys(2) 锌指蛋白，并已被定性为凋亡和坏死细胞死亡的抑制剂。为了阐明其作用的分子机制，我们使用基于酵母的双杂交系统筛选A20相关蛋白，在这里我们报道A20能够自缔合，并证明后者的相互作用是由其锌指结构域介导的。
A20 is a primary response gene which is induced after monocyte adherence or cytokine stimulation of a variety of cells, The A20 protein belongs to a novel class of Cys(2)/Cys(2) zinc finger proteins, and has been characterized as an inhibitor of both apoptotic and necrotic cell death. In order to clarify its molecular mechanism of action, we used the yeast-based two-hybrid system to screen for A20-associated proteins, Here we report that A20 is able to self-associate, and demonstrate that the latter interaction is mediated by its zinc finger domain.