Zebrafish acvr2a and acvr2b exhibit distinct roles in craniofacial development
Zebrafish acvr2a and acvr2b exhibit distinct roles in craniofacial development
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DOI：
10.1002/dvdy.20480
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发表时间：
2005-08-01
影响因子：
2.5
通讯作者：
Yelick, PC
中科院分区：
文献类型：
--
作者：
Albertson, RC;Payne-Ferreira, TL;Yelick, PC
为了研究激活素 11 型受体信号传导在颅面发育中的作用，分离了全长斑马鱼 acvr2a 和 aevr2b 克隆。尽管 acvr2a 和 acvr2b 在早期胚胎发生中普遍表达为母体 mRNA，但到受精后 24 小时 (hpf) 时，acvr2a 和 acvr2b 在神经细胞、后脑细胞和神经嵴细胞 (NCC) 中表现出有限的表达。使用基于吗啉代的靶向蛋白去除方法来揭示每个 acvr2 基因产物的离散功能。 acvr2a 变形体在大多数颅内 NCC 衍生的软骨、骨和咽齿结构的发育中表现出缺陷，而 acvr2b 变形体缺陷主要限于后牙弓结构，包括后 NCC 流的缺失和/或异常迁移、NCC 衍生的后牙弓软骨的缺陷和畸形的咽牙发育。这些研究揭示了 acvr2a 和 acvr2b 在后脑和 NCC 模式、NCC 衍生的咽弓软骨和关节形成以及牙齿发育中的先前未表征的作用。 (c) 2005 年 Wiley-Liss, Inc.
To examine the roles of activin type 11 receptor signaling in craniofacial development, full-length zebrafish acvr2a and aevr2b clones were isolated. Although ubiquitously expressed as maternal mRNAs and in early embryogenesis, by 24 hr postfertilization (hpf), acvr2a and acvr2b exhibit restricted expression in neural, hindbrain, and neural crest cells (NCCs). A morpholino-based targeted protein depletion approach was used to reveal discrete functions for each acvr2 gene product. The acvr2a morphants exhibited defects in the development of most cranial NCC-derived cartilage, bone, and pharyngeal tooth structures, whereas acvr2b morphant defects were largely restricted to posterior arch structures and included the absence and/or aberrant migration of posterior NCC streams, defects in NCC-derived posterior arch cartilages, and dysmorphic pharyngeal tooth development. These studies revealed previously uncharacterized roles for acvr2a and acvr2b in hindbrain and NCC patterning, in NCC derived pharyngeal arch cartilage and joint formation, and in tooth development. (c) 2005 Wiley-Liss, Inc.