Random cell movement promotes synchronization of the segmentation clock
Random cell movement promotes synchronization of the segmentation clock
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DOI：
10.1073/pnas.0907122107
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发表时间：
2010-03-16
影响因子：
11.1
通讯作者：
Iwasa, Yoh
中科院分区：
文献类型：
--
作者：
Uriu, Koichiro;Morishita, Yoshihiro;Iwasa, Yoh
在脊椎动物的体节发生中，体节时钟基因的表达是振荡的，并且这种振荡在附近的细胞中是同步的。实验和理论研究都表明，细胞间的同步是通过Delta-Notch信号传导的细胞间相互作用实现的。然而，出现了以下问题：（i）在体节发生过程中，动态重排的相对细胞位置中观察到的后部preomitic中胚层。在随机细胞运动下，同步状态能否稳定维持？(ii)实验研究已经报道，在完全失去同步后，细胞的同步可以在大约10个或更少的振荡周期内恢复。然而，根据先前的理论模型，这样快速的同步恢复是不可能的。在本文中，我们首先通过数值模拟表明，同步振荡下可以维持随机细胞运动。我们还发现，对于初始扰动，细胞的同步恢复得更快，它是一个更广泛的反应参数比没有细胞运动的情况下。当后前体中胚层是矩形的，更快的同步实现，如果细胞交换它们的位置与邻居位于沿着较长的一面的域。最后，我们讨论了增强同步的随机细胞运动发生在几个不同的模型的振荡分割时钟基因。
In vertebrate somitogenesis, the expression of segmentation clock genes oscillates and the oscillation is synchronized over nearby cells. Both experimental and theoretical studies have shown that the synchronization among cells is realized by intercellular interaction via Delta-Notch signaling. However, the following questions emerge: (i) During somitogenesis, dynamic rearrangement of relative cell positions is observed in the posterior presomitic mesoderm. Can a synchronized state be stably sustained under random cell movement? (ii) Experimental studies have reported that the synchronization of cells can be recovered in about 10 or fewer oscillation cycles after the complete loss of synchrony. However, such a quick recovery of synchronization is not possible according to previous theoretical models. In this paper, we first show by numerical modeling that synchronized oscillation can be sustained under random cell movement. We also find that for initial perturbation, the synchronization of cells is recovered much faster and it is for a wider range of reaction parameters than the case without cell movement. When the posterior presomitic mesoderm is rectangular, faster synchronization is achieved if cells exchange their locations more with neighbors located along the longer side of the domain. Finally, we discuss that the enhancement of synchronization by random cell movement occurs in several different models for the oscillation of segmentation clock genes.