Single particle tracking and single molecule energy transfer
Single particle tracking and single molecule energy transfer
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单粒子跟踪和单分子能量转移
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
J. Michaelis
中科院分区：
文献类型：
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作者：
C. Bräuchle;D. Lamb;J. Michaelis
序言第一部分：单粒子成像和跟踪激光扫描荧光显微镜中的三维粒子跟踪介绍基于图像的单粒子跟踪方法用于单粒子跟踪的高级荧光显微镜技术双光子激发显微镜基于图像的SPT方法中的三维跟踪激光扫描显微镜仪器中的三维跟踪背景噪声同时双粒子跟踪应用：结论细胞和组织中单个分子的跟踪介绍单分子和单颗粒定位定位精度跟踪轨迹分析应用结论信使RNA在活细胞中的运输核内结构和动力学FCS和FRAP核mRNP迁移率的研究单分子和单颗粒定位mRNA分子的粒子跟踪特异性天然mRNP的单粒子跟踪天然BR 2 mRNP的体内标记展望：基于光片的单分子显微镜技术用于活细胞单分子示踪实验的无机荧光探针胶体量子点的光学性质胶体荧光量子点的合成水的表面化学将量子点与生物学连接的量子点的溶解活细胞中的单量子点跟踪实验结论和展望第二部分：纳米级单对FRET上的能量转移：荧光共振能量转移技术的应用现状与展望荧光共振能量转移技术的原理溶液中的荧光共振能量转移技术固定化分子上的荧光共振能量转移技术的发展前景单分子的交错激发介绍ALEX：操作原理MyALEX纳秒-ALEX/脉冲交错激发（PIE）msALEX三色ALEX结论和展望揭示蛋白质结构和功能的分子结构和功能介绍将化学能转换为机械功：分子马达在蛋白质酶催化中的应用结论使用单分子FRET的定量距离和位置测量FRET的基本原理FRET作为光谱标尺：测量量子产率的初步实验和局限性供体和受体分子的取向使用荧光相关光谱法精确的FRET测量基于三角测量和纳米定位系统的结论和展望第三部分：纳米体系中的单分子单偶极发射体与其纳米环境的相干和非相干耦合引入体系两个振荡偶极的耦合靠近表面的偶极单分子和单纳米粒子自发辐射和量子的修饰结论共轭聚合物单链中的能量传递简介为什么选择单链光谱？实验方法和材料体系单共轭高分子链的光物理单链中的能量传递初始激发能对能量传递的影响结论单分子水平的反应引言单分子水平的生物催化单分子水平的化学催化单分子水平的可视化纳米通道体系中的分子扩散引言用TEM和SMT相混合物非均相动力学研究纳米通道体系的结构和动力学性质单分子取向的单分子在长的一维纳米通道中具有可切换的迁移率单分子的高定位精度低至单通道极限使用荧光相关光谱（FCS）探测二氧化硅薄膜中的化学相互作用官能化介孔二氧化硅结构用于药物递送应用的介孔二氧化硅结构的单分子研究结论和展望
Preface PART I: Single-Particle Imaging and Tracking THREE DIMENSIONAL PARTICLE TRACKING IN A LASER SCANNING FLUORESCENCE MICROSCOPE Introduction Image-Based Single-Particle Tracking Methods Advanced Fluorescence Microscopy Techniques for Single-Particle Tracking Two-Photon Excitation Microscopy 3-D Tracking in Image-Based SPT Approaches 3-D Tracking in Laser Scanning Microscopes Instrumentation Background Noise Simultaneous Two-Particle Tracking Application: Chromatin Dynamics in Interphase Cells Conclusions THE TRACKING OF INDIVIDUAL MOLECULES IN CELLS AND TISSUES Introduction Single-Molecule and Single-Particle Localization Positional Accuracy Tracking Trajectory Analysis Applications Conclusions MESSENGER RNA TRAFFICKING IN LIVING CELLS Intranuclear Structure and Dynamics FCS and FRAP Studies of Nuclear mRNP Mobility Single-Particle Tracking of mRNA Molecules Single-Particle Tracking of Specific, Native mRNPs In Vivo Labeling of Native BR2 mRNPs Outlook: Light Sheet-Based Single-Molecule Microscopy QUANTUM DOTS: INORGANIC FLUORESCENT PROBES FOR SINGLE-MOLECULE TRACKING EXPERIMENTS IN LIVE CELLS Introduction Fluorescent Labels for Single-Molecule Tracking in Cells Optical Properties of Colloidal Quantum Dots Synthesis of Colloidal Fluorescent Quantum Dots Surface Chemistry for the Water-Solubilization of Quantum Dots Interfacing Quantum Dots with Biology Single Quantum Dot Tracking Experiments in Live Cells Conclusions and Perspectives PART II: Energy Transfer on the Nanoscale SINGLE-PAIR FRET: AN OVERVIEW WITH RECENT APPLICATIONS AND FUTURE PERSPECTIVES Introduction Principles of FRET spFRET in Solution spFRET on Immobilized Molecules Future Prospects ALTERNATING-LASER EXCITATION AND PULSED-INTERLEAVED EXCITATION OF SINGLE MOLECULES Introduction ALEX: The Principles of Operation MyALEX Nanosecond-ALEX/Pulsed Interleaved Excitation (PIE) msALEX Three-Color ALEX Conclusions and Outlook UNRAVELING THE DYNAMICS BRDIGING PROTEIN STRUCTURE AND FUNCTION ONE MOLECULE AT A TIME Introduction Converting Chemical Energy to Mechanical Work: Molecular Motors Allostery in Proteins Enzyme Catalysis Conclusions QUANTITATIVE DISTANCE AND POSITION MEASUREMENT USING SINGLE-MOLECULE FRET Introduction Fundamentals of FRET FRET as a Spectroscopic Ruler: Initial Experiments and Limitations Measuring the Quantum Yield The Orientation of Donor and Acceptor Molecules Accurate FRET Measurements Using Fluorescence Correlation Spectroscopy FRET-Based Triangulation and the Nanopositioning System Conclusions and Outlook PART III: Single Molecules in Nanosystems COHERENT AND INCOHERENT COUPLING BETWEEN A SINGLE DIPOLAR EMITTER AND ITS NANOENVIRONMENT Introduction Systems Coupling of Two Oscillating Dipoles A Dipole Close to a Surface A Single Molecule and a Single Nanoparticle Modification of the Spontaneous Emission and Quantum Efficiency by Nanoantennae Conclusions ENERGY TRANSFER IN SINGLE CONJUGATED POLYMER CHAINS Introduction Why Single Chain Spectroscopy? Experimental Approach and Material Systems Photophysics of Single Conjugated Polymer Chains Energy Transfer in Single Chains Influence of Initial Excitation Energy on Energy Transfer Conclusions REACTIONS AT THE SINGLE-MOLECULE LEVEL Introduction Biocatalysis at the Single-Molecule Level Chemocatalysis at the Single-Molecule Level VISUALIZING SINGLE-MOLECULE DIFFUSION IN NANOCHANNEL SYSTEMS Introduction Correlation of Structural and Dynamic Properties Using TEM and SMT Phase Mixture Heterogeneous Dynamics of a Single Molecule Oriented Single Molecules with Switchable Mobility in Long Unidimensional Nanochannels High Localization Accuracy of Single Molecules Down to the Single Channel Limit Probing Chemical Interactions in Silica Thin Films Using Fluorescence Correlation Spectroscopy (FCS) Functionalized Mesoporous Silica Structures Single-Molecule Studies of Mesoporous Silica Structures for Drug-Delivery Applications Conclusions and Outlook