In vitro bioassay for the melanocyte stimulating hormone.
In vitro bioassay for the melanocyte stimulating hormone.
复制标题
黑素细胞刺激激素的体外生物测定。
DOI：
10.1210/endo-54-5-553
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发表时间：
1954
期刊：
影响因子：
4.8
通讯作者：
T. Fitzpatrick
中科院分区：
文献类型：
--
作者：
K. Shizume;A. Lerner;T. Fitzpatrick
1919年，阿特维尔发现，哺乳动物脑下垂体的提取物会使切除脑垂体区的蝌蚪的皮肤变暗。从那时起，这种被认为是一种激素的活性原理，一直被称为中介素、黑素载体扩张原理、黑素载体激素等，指的是它起源于脑下垂体中间叶或其作用部位，即两栖动物的黑素载体。由于黑素细胞(Fitzpatrick and Lerner，1953，Gordon，1953)一词最近被用作所有动物的黑色素形成细胞的名称，而且哺乳动物和两栖动物的黑素细胞都受到脑下垂体色素细胞激活原理的影响，后者被命名为黑素细胞刺激素(MSH)。大多数早期的测定MSH的方法包括观察注射了激素的青蛙或鱼的体内变暗。最近，Friedin，Fishbein和Hisaw(1948)描述了一种体外实验，在该实验中，孤立的青蛙变暗
In 1919 Atwell showed that extracts from mammalian pituitary glands darkened the skin of hypophysectomized tadpoles. Since then the active principle, considered a hormone, has been known as intermedin, melanophore dilating principle, melanophore hormone, etc., with reference to its source in the intermediate lobe of the pituitary gland or its site of action, the melanophores of amphibia. Because the term melanocyte (Fitzpatrick and Lerner, 1953, Gordon, 1953) recently was adopted as the name for the melanin forming cell in all animals, and because mammalian as well as amphibian melanocytes are affected by the pigment-cell activating principle of the pituitary gland, the latter has been designated as melanocyte stimulating hormone (MSH). Most of the early methods for determining MSH consisted of observing in vivo darkening of frogs or fish injected with the hormone. Recently Friedin, Fishbein, and Hisaw (1948) described an in vitro assay in which the darkening of isolated frog