Generation of peripheral B cells occurs via two spatially and temporally distinct pathways
Generation of peripheral B cells occurs via two spatially and temporally distinct pathways
复制标题
DOI：
10.1182/blood-2006-04-018085
复制
发表时间：
2007-03-15
期刊：
影响因子：
20.3
通讯作者：
Allman, David
中科院分区：
文献类型：
--
作者：
Lindsley, Robert Coleman;Thomas, Matthew;Allman, David
我们已经确定了一个新形成的骨髓（BM） B细胞群体，它们与脾脏中晚期移行B细胞具有多种特征。脾脏晚期移行性B细胞和该未表征BM群体中的细胞均表达细胞表面表型AA4(+) CD23(+)，但AA4(+) CD23(+) BM B细胞的发育动力学和更新率反映了BM和脾脏中最近最不成熟的B细胞，AA4(+) CD23(+) BM B细胞表达归巢受体CD62L，依赖于凋亡细胞因子受体BR3和tec家族激酶Btk，并在IL-4 + CD40刺激下增殖。最后，在BM和脾脏的AA4(+) CD23(+) B细胞中，lambda轻链阳性B细胞的频率下降，表明v -基因选择事件与两个区室中的CD23表达相关。这些观察结果表明，B细胞成熟的第一步发生在骨髓和外周，并表明新近形成的B细胞作为一个不成熟和半成熟B细胞的异质池退出骨髓。
We have identified a population of newly formed bone marrow (BM) B cells that shares multiple characteristics with late transitional B cells in the spleen. Both late splenic transitional B cells and cells within this uncharacterized BM population expressed the cell-surface phenotype AA4(+) CD23(+), yet the developmental kinetics and the renewal rate of AA4(+) CD23(+) BM B cells mirrored recently least mature B cells in the BM and spleen, AA4(+) CD23(+) BM B cells expressed the homing receptor CD62L, were dependent on the antlapoptotic cytokine receptor BR3 and the tec family kinase Btk, and proliferated in response to IL-4 plus CD40 stimulation. Finally, frequencies of lambda light chain-positive B cells declined among AA4(+) CD23(+) B cells in both the BM and spleen, suggesting that V-gene selection events correlate with CD23 expression in both compartments. These observations indicate that the first step in B-cell maturation occurs in both the BM and the periphery and suggest that recently formed B cells exit the BM as a heterogeneous pool of immature and semimature B cells.