A Region of Tapasin That Affects Ld Binding and Assembly1
A Region of Tapasin That Affects Ld Binding and Assembly1
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影响 Ld 结合和组装的 Tapasin 区域1
DOI：
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发表时间：
2001
影响因子：
4.4
通讯作者：
Joyce C. Solheim
中科院分区：
文献类型：
--
作者：
H. Turnquist;S. E. Vargas;A. Reber;M. McIlhaney;Suling Li;Ping Wang;S. Sanderson;B. Gubler;P. van Endert;Joyce C. Solheim
Tapasin已显示稳定TAP并将TAP连接至MHC I类H链。也有证据表明，tapasin影响肽加载到MHC I类上。为了探索tapasin与TAP和MHC I类H链结合的能力与tapasin促进I类组装的能力之间的关系，我们创建了新型tapasin突变体并在721.220-Ld细胞中表达它们。一个突变体具有9个氨基酸残基的缺失（tapasin Δ334-342），另一个在位置334和335处具有氨基酸取代。在这份报告中，我们描述了这些突变体的能力，与LD和LD表面表达的影响相互作用。我们发现Tapasin Δ334-342不能与Ld H链结合，但它促进了Ld的组装和表达。Tapasin Δ334-342能够结合并稳定TAP，表明TAP稳定化可能对Ld的组装很重要。与Tapasin Δ 334-342不同，Tapasin突变体H334 F/H335 Y结合Ld。Tapasin H334 F/H335 Y在721.220-Ld中的表达降低了Ld的细胞表面开放形式的比例，并延迟了Ld从内质网的迁移。总的来说，我们的研究结果表明，334-342区域的tapasin的影响Ld的组装和运输。
Tapasin has been shown to stabilize TAP and to link TAP to the MHC class I H chain. Evidence also has been presented that tapasin influences the loading of peptides onto MHC class I. To explore the relationship between the ability of tapasin to bind to TAP and the MHC class I H chain and the ability of tapasin to facilitate class I assembly, we have created novel tapasin mutants and expressed them in 721.220-Ld cells. One mutant has a deletion of nine amino acid residues (tapasin Δ334–342), and the other has amino acid substitutions at positions 334 and 335. In this report we describe the ability of these mutants to interact with Ld and their effects on Ld surface expression. We found that tapasin Δ334–342 was unable to bind to the Ld H chain, and yet it facilitated Ld assembly and expression. Tapasin Δ334–342 was able to bind and stabilize TAP, suggesting that TAP stabilization may be important to the assembly of Ld. Tapasin mutant H334F/H335Y, unlike tapasin Δ334–342, bound to Ld. Expression of tapasin H334F/H335Y in 721.220-Ld reduced the proportion of cell surface open forms of Ld and retarded the migration of Ld from the endoplasmic reticulum. In total, our results indicate that the 334–342 region of tapasin influences Ld assembly and transport.
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影响因子：
4.4
作者：
Beatriz M. Carreno;J. Solheim;M. Harris;Iwona T. Stroynowski;Janet M. Connolly;Ted H. Hansen
通讯作者：
Beatriz M. Carreno;J. Solheim;M. Harris;Iwona T. Stroynowski;Janet M. Connolly;Ted H. Hansen
影响因子：
4.4
作者：
J. Solheim;M. Harris;C. Kindle;Ted H. Hansen
通讯作者：
J. Solheim;M. Harris;C. Kindle;Ted H. Hansen
影响因子：
4.4
作者：
M. Harris;Y. Y. Yu-Y.;C. Kindle;Ted H. Hansen;J. Solheim
通讯作者：
M. Harris;Y. Y. Yu-Y.;C. Kindle;Ted H. Hansen;J. Solheim
HLA-B15 肽配体优先锚定在其 C 末端。
DOI：
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发表时间：
1999
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Prilliman,KR;Jackson,KW;Lindsey,M;Wang,J;Crawford,D;Hildebrand,WH
通讯作者：
Hildebrand,WH
影响因子：
4.4
作者：
J. Solheim;B. Carreno;J. D. Smith;J. Gorka;N. Myers;Zilong Wen;J. Martinko;David R. Lee;T. Hansen
通讯作者：
J. Solheim;B. Carreno;J. D. Smith;J. Gorka;N. Myers;Zilong Wen;J. Martinko;David R. Lee;T. Hansen