Validity of a self-administered food frequency questionnaire in the estimation of heterocyclic aromatic amines
Validity of a self-administered food frequency questionnaire in the estimation of heterocyclic aromatic amines
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DOI：
10.1007/s10552-014-0401-7
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发表时间：
2014-08-01
影响因子：
2.3
通讯作者：
Tsugane, Shoichiro
中科院分区：
文献类型：
--
作者：
Iwasaki, Motoki;Mukai, Tomomi;Tsugane, Shoichiro
要澄清杂环芳香胺 (HAA) 在癌症发展中的假定病因作用，需要一种经过验证的膳食 HAA 评估工具。本研究主要旨在评估食物频率问卷 (FFQ) 在估计 HAA 摄入量方面的有效性，使用人类头发中的 2-氨基-1-甲基-6-苯基咪唑并[4,5-b]吡啶 (PhIP) 水平作为参考方法。我们首先使用液相色谱-电喷雾电离/串联质谱 (LC-ESI/MS/MS) 更新了 PhIP 的分析方法，并测量了来自喂养研究的 9 只大鼠的 44 个毛皮样本作为部分验证LC-ESI/MS/MS 的定量性能。接下来，我们通过 FFQ 验证研究测量了人类头发样本中的 PhIP 水平 (n = 65)。使用六种鱼类和七种肉类的摄入量信息以及每种食品中 HAA 含量的数据估算了 FFQ 中的 HAA 摄入量。计算了人头发中PhIP水平与FFQ中HAA摄入量之间的相关系数。PhIP的动物喂养研究发现，剂量与大鼠毛皮中PhIP之间存在显着的剂量反应关系。在数值超过检测限 (LOD) 的受试者中，平均水平为 53.8 皮克/克头发 (n = 57)。我们发现人发中的 PhIP 与 FFQ 中的 HAA 摄入量之间存在显着的正相关系数，所有受试者的 Spearman 等级相关系数为 0.35，超过 LOD 值的受试者为 0.21，超过定量限制的受试者为 0.34。验证研究的结果表明，FFQ 对于评估 HAA 摄入量是合理有效的。
Clarification of the putative etiologic role of heterocyclic aromatic amines (HAAs) in the development of cancer requires a validated assessment tool for dietary HAAs. This study primarily aimed to evaluate the validity of a food frequency questionnaire (FFQ) in estimating HAA intake, using 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) level in human hair as the reference method.We first updated analytical methods of PhIP using liquid chromatography-electrospray ionization/tandem mass spectrometry (LC-ESI/MS/MS) and measured 44 fur samples from nine rats from a feeding study as part-verification of the quantitative performance of LC-ESI/MS/MS. We next measured PhIP level in human hair samples from a validation study of the FFQ (n = 65). HAA intake from the FFQ was estimated using information on intake from six fish items and seven meat items and data on HAA content in each food item. Correlation coefficients between PhIP level in human hair and HAA intake from the FFQ were calculated.The animal feeding study of PhIP found a significant dose-response relationship between dosage and PhIP in rat fur. Mean level was 53.8 pg/g hair among subjects with values over the limit of detection (LOD) (n = 57). We found significant positive correlation coefficients between PhIP in human hair and HAA intake from the FFQ, with Spearman rank correlation coefficients of 0.35 for all subjects, 0.21 for subjects with over LOD values, and 0.34 for subjects with over limit of quantification.Findings from the validation study suggest that the FFQ is reasonably valid for the assessment of HAA intake.