Silicon and phosphorus growth kinetics and competitive interactions between Stephanodiscus minutes and Synedra sp.: With 1 figure and 1 table in the text
Silicon and phosphorus growth kinetics and competitive interactions between Stephanodiscus minutes and Synedra sp.: With 1 figure and 1 table in the text
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硅和磷的生长动力学以及 Stephanodiscus 分钟和 Synedra sp. 之间的竞争相互作用：文中附有 1 个图和 1 个表
DOI：
10.1080/03680770.1983.11897326
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发表时间：
1984
期刊：
影响因子：
1.4
通讯作者：
S. S. Kilham
中科院分区：
文献类型：
--
作者：
S. S. Kilham
方法从DoNAI附近的密歇根湖南岸近岸水样中分离硅藻。D e. 1977年10月，库克发电厂。微小冠盘藻（Stephanodiscus minutus，克隆LMSTEPH S1 JM）是由J. A.和针杆藻属（Synedra sp.）（克隆LM SYN）由SS Kn分离。HAM使用无菌移液管技术（GUILLAJ. D 1973）。一般培养条件在MEcHLING & Kn中给出。HAM（1982），以及分批培养MoNoo实验和连续D&OOP实验的描述。对于在三个温度（10、15、20 ℃）下硅（Si）限制的针杆藻属的MoNOD实验，烧瓶含有浓度范围为0.4 - 22 μ Si的介质。在20 oe. Synedra细胞没有预处理，指数生长的培养物被用作接种物。对于两个物种的MoNOD实验，在磷（P）限制下，Stephanodiscus为10和20 oe，Synedra为20 oe，培养瓶中含有浓度为0.05 - 5.5 μ P的培养基。通过将指数生长的细胞转移到”无P”培养基中来预处理两种细胞（通常具有约0.05~ P），并使它们生长5天，然后接种到实验烧瓶中。AllP限制培养基添加KHC 03以将K水平维持在100 ° K。针杆藻连续培养D&ooP实验中，在20 oe，2个组合实验的进水浓度分别为11.0和10.7~ Si，在10 g 0 ℃，实验的进水浓度为9.6~ Si。在限磷条件下，针杆藻的进水磷浓度分别为0.26~ P（20 ℃）和0.32~ P（10 ℃），冠藻的进水磷浓度分别为1.2~ P（20 ℃）和1.7~ P（10 ℃）。竞争实验在125 m1聚碳酸酯ElU中进行。我啊！NMEYELl烧瓶中使用连续技术。体积为80 ml，取出20 ml培养物悬浮液，每天一次用无菌培养基替换（流量= 0.25天-1;生长= 0.29天-1）。在10 e时，进水硅、磷浓度分别为：5.69~ 5.28~ 5.28（Si：P= 1.1）; 65.04~ Si，12.23~ P（Si：P= 5.3）; 53.28~ Si，5.11~ P（Si：P= 10.4）; 56.15~ Si，2.42~ P（Si：P = 23.2）; 52.19~ Si，1.60~ P（Si：P= 32.6）; 53.46~ Si，0.48~ P（Si：P= 111）。的
MethodsThe diatoms were isolated from a nearshore southem Lake Michigan water sample taken in the vicinity of the DoNAI. D e. CooK Power Plant in October 1977. Stephanodiscus minutus (clone LM STEPH Sl JM) was isolated by J. A. MEcHLING, and Synedra sp.(clone LM SYN) was isolated by SS Kn. HAM using a sterile pipet technique (GUILLAJ. D 1973). General culture conditions are given in MEcHLING & Kn. HAM (1982), as well as a description of the batch culture MoNoo experiments and the semicontinuous D&OOP experiments. For the MoNOD experiments for Sy-nedra sp. limited by silicon (Si) at three temperatures (10, 15, 20 C}, the flasks contained media ranging in concentration from 0.4 to 22~ Si. Two experiments six months apart were performed at 20 oe. The Synedra cells were not preconditioned; exponentially growing cultures were used as inocula. For the MoNOD experiments for both species limited by phosphorus (P) at lO and 20 oe for Stephanodiscus and 20 oe for Synedra, the flasks contained media ranging in concentration from 0.05 to 5.5~ P. eells of both species were preconditioned by transferring exponentially growing cells into" P-free" medium (usually with about 0.05~ P) and allowing them to grow for five days prior to inoculation into the experimental flasks. A11 P-limited media had KHC03 added to maintain the K level at 100~ K. For the semicontinuous culture D&ooP experiments under Si Iimitation for Synedra, the influent concentrations were 11.0 and 10.7~ Si for the two combined experiments at 20 oe, and 9.6~ Si for the experiments at 10g 0 C. For the D&ooP experiments under P limitation, the influent P concentrations were 0.26~ P (20 C) and 0.32~ P (10 C) for Synedra, and 1.2~ P (20 C) and 1.7~ P(lO 0 C) for Stephanodiscus. The competition experiments were performed in 125 m1 polycarbonate ElU. I! NMEYEll flasks using the semicontinuous technique. The volume was 80 ml, and 20 m1 of culture suspension was removed and replaced by sterile medium once each day (flow= 0.25 day-1; growth-0.29 day-1). The influent Si and P concentrations were as follows at l0e: 5.69~ Si, 5.28~ P (Si: P= 1.1); 65.04~ Si, 12.23~ P (Si: P= 5.3); 53.28~ Si, 5.11~ P (Si: P= 10.4); 56.15~ Si, 2.42~ P (Si: P-23.2}; 52.19~ Si, 1.60~ P (Si: P= 32.6}; 53.46~ Si, 0.48~ P (Si: P= 111). The