The detection of non-RoTat 1.2 Trypanosoma evansi
The detection of non-RoTat 1.2 Trypanosoma evansi
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DOI：
10.1016/j.exppara.2005.01.001
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发表时间：
2005-05-01
影响因子：
2.1
通讯作者：
Ngeranwa, JJN
中科院分区：
文献类型：
--
作者：
Ngaira, JM;Olembo, NK;Ngeranwa, JJN
伊氏锥虫Rode锥虫抗原型（RoTat）1.2. T是一个数。伊文思在肯尼亚被隔离。对T. evansi的VSG基因，克隆并测序了其cDNA序列。evansi JN 2118Hu，一个缺乏RoTat 1.2 VSG基因的分离物。以VSG基因的273 bp DNA片段为靶点进行PCR扩增，检测非RoTat 1.2 T。evansi对32株锥虫的基因组DNA进行了检测。伊氏锥虫、布氏锥虫10只、刚果锥虫3只、间日锥虫1只。通过PCR扩增RoTat 1.2 VSG基因的488 bp片段进行比较。结果表明，预期的273 bp扩增产物存在于所有五个非RoTat 1.2 T。evansi检测，27只RoTat 1.2阳性T.埃文斯测试。在所有其他测试的锥虫中也不存在。本研究中开发的PCR检测对非RoTat 1.2 T具有特异性。evansi (c)2005年爱思唯尔公司All rights reserved.
Trypanosoma evansi Rode Trypanozoon antigen type (RoTat) 1.2. Exceptions are a number of T. evansi isolated in Kenya. To characterize T. evansi that are undetected by RoTat 1.2, we cloned and sequenced the VSG cDNA from T. evansi JN 2118Hu, an isolate devoid of the RoTat 1.2 VSG gene. A 273 bp DNA segment of the VSG gene was targeted in PCR amplification for the detection of non-RoTat 1.2 T. evansi. Genomic DNA samples from different trypanosomes were tested including 32 T. evansi, 10 Trypanosoma brucei, three Trypanosoma congolense, and one Trypanosoma vivax. Comparison was by PCR amplification of a 488 bp fragment of RoTat1.2 VSG gene. Results showed that the expected 273 bp amplification product was present in all five non-RoTat 1.2 T. evansi tested and was absent in all 27 RoTat 1.2-positive T. evansi tested. It was also absent in all other trypanosomes tested. The PCR test developed in this study is specific for non-RoTat 1.2 T. evansi. (c) 2005 Elsevier Inc. All rights reserved.