Histopathological evaluation including cytokeratin 13 and Ki-67 in the border between Lugol-stained and -unstained areas
Histopathological evaluation including cytokeratin 13 and Ki-67 in the border between Lugol-stained and -unstained areas
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DOI：
10.3892/or_00000822
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发表时间：
2010-07-01
期刊：
影响因子：
4.2
通讯作者：
Kamata, Nobuyuki
中科院分区：
文献类型：
--
作者：
Ohta, Kouji;Ogawa, Ikuko;Kamata, Nobuyuki
卢戈氏碘染色（卢戈染色）已被广泛用于检测子宫颈和食管的恶性变化。然而，病理和组织化学变化之间的边界卢戈尔染色和非染色地区的口腔上皮发育不良和恶性病变还没有得到很好的理解。我们研究了20例鳞状细胞癌周围上皮异型增生的组织学表现，在Lugol染色和未染色区域之间的边界使用HE和PAS染色。随后，通过免疫组织化学研究了这些区域中细胞角蛋白13（CK 13）（上皮分化标记物）和Ki-67（细胞增殖标记物）表达的差异。所有病例的Lugol染色区域均显示轻度不典型增生或正常上皮外观，而Lugol未染色区域大多数病例被诊断为中/重度不典型增生和原位癌。PAS反应在Lugol未染色区域中有限或未发现，而在Lugol染色区域中为强阳性。CK 13和Ki-67蛋白表达在Lugol染色和未染色区域之间有显著差异。证实了显示癌前或癌特征的上皮被检测为Lugol未染色的边界区域。与恶性病变相关的细胞分化和增殖改变引起的糖原产生减少可能导致Lugol染色缺乏。
Lugol's iodine staining (Lugol-staining) has been widely used to detect malignant changes in the cervix uteri and esophagus. However, pathological and histochemical changes in the border between Lugol-stained and -unstained areas in oral epithelial dysplastia and malignant lesions are not well understood. We examined the histological appearance of 20 cases of epithelial dysplasia surrounding squamous cell carcinoma using HE and PAS staining in the borders between Lugol-stained and -unstained areas. Subsequently, differences in the expression of cytokeratin 13 (CK13), an epithelium differentiation marker, and Ki-67, a cell proliferative marker, in those areas were investigated by immunohistochemistry. Lugol-stained areas of all cases showed mild dysplasia or normal epithelium appearance, while Lugol-unstained areas in most cases were diagnosed as moderate/severe dysplasia and carcinoma in situ. PAS reactions were limited or not found in the Lugol-unstained areas as compared to intense positivity in Lugol-stained areas. CK13 and Ki-67 protein expression was significantly different between Lugol-stained and -unstained areas. It was confirmed that epithelia showing precancerous or cancerous features were detected as Lugol-unstained boundary areas. A reduction in glycogen production caused by alterations of cell differentiation and proliferation associated with malignant changes may result in a lack of Lugol-staining.