Multispectral Photoacoustic Imaging of Prostate Cancer: Preliminary Ex-vivo Results.
Multispectral Photoacoustic Imaging of Prostate Cancer: Preliminary Ex-vivo Results.
复制标题
DOI：
10.4103/2156-7514.119139
复制
发表时间：
2013
影响因子：
0.9
通讯作者：
Rao NA
中科院分区：
文献类型：
--
作者：
Dogra VS;Chinni BK;Valluru KS;Joseph JV;Ghazi A;Yao JL;Evans K;Messing EM;Rao NA
本研究的目的是验证离体多光谱光声（PA）成像是否可以区分恶性前列腺组织、良性前列腺增生（BPH）和正常人类前列腺组织。本研究已获得机构审查委员会的批准。本研究共纳入 30 名因活检确诊前列腺癌而接受前列腺切除术的患者，并获得知情同意。对手术切除的前列腺组织进行多光谱 PA 成像，并重建代表脱氧血红蛋白 (dHb)、氧合血红蛋白 (HbO2)、脂质和水的光学吸收的发色团图像。成像过程完成后，泌尿生殖病理学家在组织病理学载玻片上标记恶性前列腺、BPH和正常前列腺区域，并获得标记的组织病理学载玻片的数字图像。组织病理学图像与发色团图像共同配准。在发色团图像上定义对应于恶性前列腺、BPH 和正常前列腺的感兴趣区域 (ROI)。然后对每个 ROI 内的像素值进行平均，以确定 dHb、HbO2、脂质和水的平均强度。我们的初步结果表明，恶性前列腺组织和正常前列腺组织之间的 dHb 平均强度（P < 0.0001）和脂质平均强度（P = 0.0251）存在统计学显着差异。恶性前列腺与BPH之间dHb平均强度存在差异（P<0.0001）。我们的成像系统的敏感性、特异性、阳性预测值和阴性预测值分别为 81.3%、96.2%、92.9% 和 89.3%。我们对离体人类前列腺研究的初步结果表明，多光谱 PA 成像可以区分恶性前列腺、BPH 和正常前列腺组织。
The objective of this study is to validate if ex-vivo multispectral photoacoustic (PA) imaging can differentiate between malignant prostate tissue, benign prostatic hyperplasia (BPH), and normal human prostate tissue. Institutional Review Board's approval was obtained for this study. A total of 30 patients undergoing prostatectomy for biopsy-confirmed prostate cancer were included in this study with informed consent. Multispectral PA imaging was performed on surgically excised prostate tissue and chromophore images that represent optical absorption of deoxyhemoglobin (dHb), oxyhemoglobin (HbO2), lipid, and water were reconstructed. After the imaging procedure is completed, malignant prostate, BPH and normal prostate regions were marked by the genitourinary pathologist on histopathology slides and digital images of marked histopathology slides were obtained. The histopathology images were co-registered with chromophore images. Region of interest (ROI) corresponding to malignant prostate, BPH and normal prostate were defined on the chromophore images. Pixel values within each ROI were then averaged to determine mean intensities of dHb, HbO2, lipid, and water. Our preliminary results show that there is statistically significant difference in mean intensity of dHb (P < 0.0001) and lipid (P = 0.0251) between malignant prostate and normal prostate tissue. There was difference in mean intensity of dHb (P < 0.0001) between malignant prostate and BPH. Sensitivity, specificity, positive predictive value, and negative predictive value of our imaging system were found to be 81.3%, 96.2%, 92.9% and 89.3% respectively. Our preliminary results of ex-vivo human prostate study suggest that multispectral PA imaging can differentiate between malignant prostate, BPH and normal prostate tissue.