Interleukin 12 is a primary cytokine responding to influenza virus infection in the respiratory tract of mice
Interleukin 12 is a primary cytokine responding to influenza virus infection in the respiratory tract of mice
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DOI：
10.4149/av_2009_04_233
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发表时间：
2009-01-01
期刊：
影响因子：
1.7
通讯作者：
Shiraki, K.
中科院分区：
文献类型：
--
作者：
Hama, Y.;Kurokawa, M.;Shiraki, K.
我们以前曾报道过，感染甲型流感病毒或鼻内（i.n.）对感染的小鼠施用IL-12减轻了肺炎（Tsuritaetal.，J. Pharmacol. Exp.治疗298，362-368，2001）。在这项研究中，我们发现，在支气管肺泡灌洗液（BALF）从小鼠感染i. n。在感染后第1天（p.i.）并且随后在感染后第2天给予肿瘤坏死因子α（TNF-α）、IL-18和干扰素α、β和γ（IFN-α、IFN-β和IFN-γ）。感染后第1天感染肺的组织化学分析。结果表明，IL-12和IL-12 mRNA存在于单核细胞和巨噬细胞样细胞中，巨噬细胞与病毒抗原共定位，而其他细胞因子则不存在。因此，IL-12是由浸润感染上皮的巨噬细胞产生的第一反应细胞因子，并且其在感染部位的产生可以指导早期免疫防御以减轻感染的严重程度。
We have reported previously that an increase in interleukin 12 (IL-12) production in the lungs of mice infected with Influenza A virus or an intranasal (i.n.) administration of IL-12 to the infected mice alleviated pneumonia (Tsurita et al., J. Pharmacol. Exp. Therapeut. 298, 362-368, 2001). In this study, we found that in the bronchoalveolar lavage fluids (BALF) obtained from mice infected i.n. with Influenza A virus IL-12 was elevated on day 1 post infection (p.i.) and was followed by tumor necrosis factor alpha (TNF-alpha), IL-18, and interferons alpha, beta, and gamma (IFN-alpha, -beta, and -gamma) on day 2 p.i. Histochemical analyses of the infected lungs on day 1 p.i. showed the presence of IL-12 and IL-12 mRNA in mononuclear and macrophage-like cells and co-localization of macrophages with viral antigen, while other cytokines were absent. Thus, IL-12 was produced by macrophages infiltrating the infected epithelium as the first response cytokine and its production at the site of infection may direct an early immune defense to alleviate the severity of infection.