Transcript levels of the Aspergillus fumigatus Cdc42 module, polarisome, and septin genes show little change from dormancy to polarity establishment.
Transcript levels of the Aspergillus fumigatus Cdc42 module, polarisome, and septin genes show little change from dormancy to polarity establishment.
复制标题
烟曲霉 Cdc42 模块、极化体和 septin 基因的转录水平从休眠到极性建立几乎没有变化。
DOI：
10.1093/mmy/myw085
复制
发表时间：
2017
期刊：
影响因子：
2.9
通讯作者：
Momany,Michelle
中科院分区：
文献类型：
--
作者：
Oda,Ken;Bignell,Elaine;Kang,SEarl;Momany,Michelle
烟曲霉菌是最常见的空气传播病原体，可引起免疫功能低下患者的致命真菌病。在开发的前8个小时A。烟曲霉分生孢子打破休眠，同位素扩张，建立极性轴，并开始以极性方式延伸芽管。从各向同性到极性生长的转变对于组织侵入和发病机制是至关重要的。在目前的工作中，我们使用双色微阵列来检测A。fumigatu转录组在早期发展过程中，侧重于各向同性的极性开关。在各向同性的极性开关最高度调节的转录本不包括已知的极性基因。转录本编码Cdc42模块，polarisome组件，和septins，被称为极性的关键球员，表现出相对稳定的水平，在各向同性极性开关。事实上，这些成绩单存在于休眠分生孢子，其水平变化不大，从休眠到芽管出现。各向同性极性开关不仅在Cdc42模块、极化体和septins的关键极性基因的表达中几乎没有变化，而且在早期发育中上调和下调的总体水平也最低。
Aspergillus fumigatusis the most common airborne pathogen causing fatal mycoses in immunocompromised patients. During the first 8 hours of developmentA. fumigatusconidia break dormancy, expand isotopically, establish an axis of polarity, and begin to extend germ tubes in a polar manner. The transition from isotropic to polar growth is critical for tissue invasion and pathogenesis. In the current work, we used two-color microarrays to examine theA. fumigatustranscriptome during early development, focusing on the isotropic to polar switch. The most highly regulated transcripts in the isotropic to polar switch did not include known polarity genes. Transcripts encoding the Cdc42 module, polarisome components, and septins, known to be critical players in polarity, showed relatively steady levels during the isotropic to polar switch. Indeed, these transcripts were present in dormant conidia, and their levels changed little from dormancy through germ tube emergence. Not only did the isotropic to polar switch show little change in the expression of key polarity genes of the Cdc42 module, polarisome, and septins, it also showed the lowest overall levels of both up- and downregulation in early development.