BINDING OF MATRIX ATTACHMENT REGIONS TO LAMIN-B1
BINDING OF MATRIX ATTACHMENT REGIONS TO LAMIN-B1
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DOI：
10.1016/0092-8674(92)90245-8
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发表时间：
1992-09-18
期刊：
影响因子：
64.5
通讯作者：
VANDRIEL, R
中科院分区：
文献类型：
--
作者：
LUDERUS, MEE;DEGRAAF, A;VANDRIEL, R
真核染色质被组织成拓扑约束的环，附着在核基质上。与基质相互作用的 DNA 区域称为基质附着区域 (MAR)。我们采用生化和超微结构相结合的方法，研究了大鼠肝细胞核基质中 MAR 结合位点的空间分布。我们发现 MAR 结合位点均匀分布在内部纤维颗粒网络和外周核层上。内部和外周结合位点具有相似的结合特征：两组结合位点均显示来自不同生物体的 MAR 的特异性和可饱和结合。通过DNA结合蛋白印迹测定和体外结合研究，我们将核纤层蛋白B1鉴定为MAR结合蛋白，这为DNA与核纤层的特异性相互作用提供了证据。
Eukaryotic chromatin is organized into topologically constrained loops that are attached to the nuclear matrix. The regions of DNA that interact with the matrix are called matrix attachment regions (MARs). We studied the spatial distribution of MAR-binding sites in the nuclear matrix from rat liver cells, following a combined biochemical and ultrastructural approach. We found that MAR-binding sites are distributed equally over the internal fibrogranular network and the peripheral nuclear lamina. Internal and peripheral binding sites have similar binding characteristics: both sets of binding sites show specific and saturable binding of MARs from different organisms. By means of a DNA-binding protein blot assay and in vitro binding studies, we identified lamin B1 as a MAR-binding protein, which provides evidence for a specific interaction of DNA with the nuclear lamina.