Uncertainty from sampling in measurements of aflatoxins in animal feedingstuffs: application of the Eurachem/CITAC guidelines
Uncertainty from sampling in measurements of aflatoxins in animal feedingstuffs: application of the Eurachem/CITAC guidelines
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DOI：
10.1039/c1an15124j
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发表时间：
2011-01-01
期刊：
影响因子：
4.2
通讯作者：
Andersson, Mats Gunnar
中科院分区：
文献类型：
--
作者：
Reiter, Elisabeth Viktoria;Dutton, Mike Francis;Andersson, Mats Gunnar
Eurachem/CITAC指南中介绍了用于估计测量（包括采样）不确定度的重复方法。在三个动物饲料中黄曲霉毒素B-1的案例研究中，评估了该方法作为验证霉菌毒素采样计划工具的适用性。考虑的方面包括从受污染批次中获取样品的策略、关于分布的假设、统计分析方法、试验数据的log（10）转换和不确定性估计的适用性。结果表明，当重复骨料样品形成的穿插抽样，从一个批次的重复测量可以假定近似遵循正态分布或对数正态分布。由于采样目标之间毒素浓度的差异很大，有时采样和样品制备产生非常大的不确定性（U-rel >= 50%），因此发现从log（10）转换数据估计不确定性是比应用稳健ANOVA更普遍适用的方法。
The duplicate method for estimating uncertainty from measurement including sampling is presented in the Eurachem/CITAC guide. The applicability of this method as a tool for verifying sampling plans for mycotoxins was assessed in three case studies with aflatoxin B-1 in animal feedingstuffs. Aspects considered included strategies for obtaining samples from contaminated lots, assumptions about distributions, approaches for statistical analysis, log(10)-transformation of test data and applicability of uncertainty estimates. The results showed that when duplicate aggregate samples are formed by interpenetrating sampling, repeated measurements from a lot can be assumed to approximately follow a normal or lognormal distribution. Due to the large variation in toxin concentration between sampling targets and sometimes very large uncertainty arising from sampling and sample preparation (U-rel >= 50%), estimation of uncertainty from log(10)-transformed data was found to be a more generally applicable approach than application of robust ANOVA.