Alpha-syntrophin dependent expression of tubulin alpha 8 protein in hepatocytes
Alpha-syntrophin dependent expression of tubulin alpha 8 protein in hepatocytes
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肝细胞中微管蛋白α8蛋白的α-肌营养蛋白依赖性表达
DOI：
10.1007/s13105-018-0645-x
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发表时间：
2018
影响因子：
3.4
通讯作者：
Buechler C
中科院分区：
文献类型：
--
作者：
Rein- Fischboeck L;Bajraktari G;Pohl R;Feder S;Eisinger K;Mages W;Haberl EM;Buechler C
支架蛋白α-突触营养蛋白（SNTA）是肌营养不良蛋白糖蛋白复合物的一种组分，已在骨骼肌和脂肪细胞中进行了全面研究。SNTA在肝脏中进一步表达，其生物学作用尚不清楚。我们小组未发表的数据表明，SNTA缺乏与脂肪中微管蛋白α 8（TUBA 8）水平的改变有关。TUBA 8在包括肝癌细胞在内的不同细胞系中高度表达，在这里我们分析了SNTA是否在其中起作用。在Hepa 1 -6细胞中，TUBA 8蛋白水平在SNTA敲低后增加，在SNTA过表达后降低。在分析mRNA表达时未发现这种调节。在SNTA缺陷小鼠的肝脏中，与相应的野生型对照相比，TUBA 8蛋白质含量更高，而RNA表达甚至受到抑制。使用HaloTag平台，发现TUBA 8在Hepa 1 -6细胞中与SNTA形成复合物。然而，在肝星状细胞系LX-2中，SNTA的缺乏或过表达并不改变TUBA 8蛋白的表达。SNTA和TUBA 8被描述为调节细胞增殖。然而，敲低SNTA既不影响Hepa 1 -6细胞的增殖，也不影响细胞活力。本研究表明，SNTA蛋白水平与肝细胞系Hepa 1 -6中TUBA 8蛋白表达呈负相关。
The scaffold protein alpha-syntrophin (SNTA) is a component of the dystrophin glycoprotein complex and has been comprehensively studied in skeletal muscle and adipocytes. SNTA is further expressed in the liver where its biological role remains unclear. Unpublished data from our group suggested that SNTA deficiency is associated with altered tubulin alpha 8 (TUBA8) levels in fat. TUBA8 is highly expressed in different cell lines including hepatoma cells, and here we analyzed whether SNTA has a role herein. In Hepa1-6 cells, TUBA8 protein levels were increased upon SNTA knock down and were reduced upon overexpression of SNTA. This regulation was not identified when analyzing mRNA expression. In the liver of SNTA-deficient mice, TUBA8 protein was higher compared to the respective wild-type controls while RNA expression was even suppressed. Using the HaloTag platform, TUBA8 was found to form a complex with SNTA in Hepa1-6 cells. In the hepatic stellate cell line LX-2, the lack or overexpression of SNTA did, however, not change TUBA8 protein expression. SNTA and TUBA8 are described to regulate cell proliferation. Yet, knock down of SNTA did neither affect proliferation nor viability of Hepa1-6 cells. The present study shows that SNTA protein levels are inversely related to TUBA8 protein expression in the hepatocyte cell line Hepa1-6.
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影响因子：
7.8
作者：
Luo, Shuo;Chen, Yu;Lai, Kwok-On;Arevalo, Juan Carlos;Froehner, Stanley C;Adams, Marvin E;Chao, Moses V;Ip, Nancy Y
通讯作者：
Ip, Nancy Y
影响因子：
3.6
作者：
L. Rein‐Fischboeck;R. Pohl;E. Haberl;T. Weiss;C. Buechler
通讯作者：
L. Rein‐Fischboeck;R. Pohl;E. Haberl;T. Weiss;C. Buechler
影响因子：
11.2
作者：
Yue F;Li W;Zou J;Jiang X;Xu G;Huang H;Liu L
通讯作者：
Liu L
a1-肌营养蛋白调节 ABCA1 的周转
DOI：
--
发表时间：
2004
期刊：
J Biol Chem 279
影响因子：
--
作者：
Munehira;Y.;Ohnishi;T.;Kawamoto;S.;Furuya;A.;Shitara;K.;Imamura;M.;Yokota;T.;Takeda;S.i.;Amachi;T.;Matsuo;M.;Kioka;N.;Ueda;K
通讯作者：
K
DOI：
10.1016/j.toxicon.2011.03.008
发表时间：
2011-05
期刊：
Toxicon : official journal of the International Society on Toxinology
影响因子：
--
作者：
Yong Zhang;Guoyu Yu;Dongmei Wang;Ying Hu;W. Lei
通讯作者：
Yong Zhang;Guoyu Yu;Dongmei Wang;Ying Hu;W. Lei