Direct interaction of microtubule- and actin-based transport motors
Direct interaction of microtubule- and actin-based transport motors
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DOI：
10.1038/16722
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发表时间：
1999-01-21
期刊：
影响因子：
64.8
通讯作者：
Jenkins, NA
中科院分区：
文献类型：
--
作者：
Huang, JD;Brady, ST;Jenkins, NA
微管网络被认为用于动物细胞中细胞组分的长距离运输，而肌动蛋白网络被认为用于短距离运输（1），尽管这种运输协调的机制知之甚少。例如，在海胆中，胞吐囊泡的长距离Ca 2+调节运输需要基于微管的马达，而基于肌动蛋白的马达用于短程运输（2），在神经元中，基于微管的驱动蛋白马达蛋白用于长距离囊泡运输（3），但微管不延伸到神经元末端，其中肌动蛋白丝形成细胞骨架框架（4），而驱动蛋白在到达神经元末端后迅速降解（5），表明囊泡可能必须从微管转移到肌动蛋白轨道才能到达其最终目的地。在这里，我们表明，肌动蛋白为基础的囊泡运输马达，MyoVA（参考文献6），可以直接与微管为基础的运输马达，KhcU。如果这些复合物是功能性的，则如所预期的，它们还含有驱动蛋白轻链，并且MyoVA和KhcU在细胞中的定位重叠。这些结果表明，细胞转运部分地通过不同马达分子的直接相互作用来协调。
The microtubule network is thought to be used for long-range transport of cellular components in animal cells whereas the actin network is proposed to be used for short-range transport(1), although the mechanism(s) by which this transport is coordinated is poorly understood. For example, in sea urchins long-range Ca2+-regulated transport of exocytotic vesicles requires a microtubule-based motor, whereas an actin-based motor is used for short-range transport(2), In neurons, microtubule-based kinesin motor proteins are used for long-range vesicular transport(3) but microtubules do not extend into the neuronal termini, where actin filaments form the cytoskeletal framework(4), and kinesins are rapidly degraded upon their arrival in neuronal termini(5), indicating that vesicles may have to be transferred from microtubules to actin tracks to reach their final destination. Here we show that an actin-based vesicle-transport motor, MyoVA (ref. 6), can interact directly with a microtubule-based transport motor, KhcU. As would be expected if these complexes were functional, they also contain kinesin light chains and the localization of MyoVA and KhcU overlaps in the cell, These results indicate that cellular transport is, in part, coordinated through the direct interaction of different motor molecules.