Identification of Molecular Markers That Are Specific to the Class Thermoleophilia
Identification of Molecular Markers That Are Specific to the Class Thermoleophilia
复制标题
嗜热嗜油菌类特有的分子标记的鉴定
DOI：
10.3389/fmicb.2019.01185
复制
发表时间：
2019-05-24
影响因子：
5.2
通讯作者：
Gao, Beile
中科院分区：
文献类型：
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作者：
Hu, Danyu;Zang, Yang;Gao, Beile
嗜热菌是放线杆菌门中的一个深生根谱系，对微生物多样性的元基因组研究表明，在温泉和土壤样品中存在大量与嗜热菌有关的物种。然而，这一纲的物种很少被培养和表征。我们对嗜热杆菌的系统发育和分类的理解完全基于对有限的可培养代表的16S rRNA序列分析，但没有其他表型或基因特征可以清楚地将这一类别的成员与细菌界中的其他分类单位区分开来。这项研究报告了该纲12个已测序成员的系统基因组分析，并清楚地解决了尚未培育的物种与重建的基因组和已知模式物种的相互关系。比较基因组分析发现，在不同的蛋白质中发现了12个CSI，并发现了32个CSP，它们是这一类所有物种所特有的。此外，大量的CSIS或CSP被发现是这一类中某些谱系所独有的。本研究首次对嗜热菌进行了系统发育分析，鉴定出的CSIS和CSPs为鉴定和描述该纲或其附属类群的物种提供了有价值的分子标记。
The class Thermoleophilia is one of the deep-rooting lineages within the Actinobacteria phylum and metagenomic investigation of microbial diversity suggested that species associated with the class Thermoleophilia are abundant in hot spring and soil samples. However, very few species of this class have been cultivated and characterized. Our understanding of the phylogeny and taxonomy of Thermoleophilia is solely based on 16S rRNA sequence analysis of limited cultivable representatives, but no other phenotypic or genotypic characteristics are known that can clearly discriminate members of this class from the other taxonomic units within the kingdom bacteria. This study reports phylogenomic analysis for 12 sequenced members of this class and clearly resolves the interrelationship of not yet cultivated species with reconstructed genomes and known type species. Comparative genome analysis discovered 12 CSIs in different proteins and 32 CSPs that are specific to all species of this class. In addition, a large number of CSIs or CSPs were identified to be unique to certain lineages within this class. This study represents the first and most comprehensive phylogenetic analysis of the class Thermoleophilia, and the identified CSIs and CSPs provide valuable molecular markers for the identification and delineation of species belonging to this class or its subordinate taxa.