Peptide discovery in the ectoparasitic crustacean Argulus siamensis: Identification of the first neuropeptides from a member of the Branchiura
Peptide discovery in the ectoparasitic crustacean Argulus siamensis: Identification of the first neuropeptides from a member of the Branchiura
复制标题
DOI：
10.1016/j.ygcen.2014.05.004
复制
发表时间：
2014-08-01
影响因子：
2.7
通讯作者：
Christie, Andrew E.
中科院分区：
文献类型：
--
作者：
Christie, Andrew E.
高通量测序的最新进展促进了为越来越多的甲壳类动物生成大型转录组数据集，其中之一是鲤鱼虱（Argulus siamensis）。这种鱼和鳃亚纲的其他成员是专性鱼类体外寄生虫，因此是商业水产养殖的主要关注点。使用暹罗亚种现有的转录组鸟枪组装 (TSA) 序列，鉴定了编码推定神经肽前体的 27 个转录本，并使用完善的生物信息学工作流程推导和表征了它们的前/前激素原。从推导的蛋白质中预测了105种不同肽的结构，包括脂肪运动激素(AKH)、尿抑素A、尿抑素B、尿抑素C、Allatotropin、bursicon α、bursicon β、甲壳动物心脏活性肽(CCAP)、利尿激素31、利尿激素44、羽化激素、肌抑制素、神经节素、神经肽Y、orcokinin、色素分散激素、proctolin、短神经肽F、SIFamide、磺胺激肽和速激肽相关肽。虽然一些预测的肽是从其他甲壳类动物和/或昆虫物种中已知的，例如甲壳类动物和/或昆虫物种。 RYLPT，一种广泛保守的节肢动物原肠蛋白亚型，和 PECNAFTGCamide（两个半胱氨酸之间的二硫键桥），典型的甲壳动物 CCAP，其中绝大多数是首次在这里描述，例如pQVNESTKWamide，一种新的 AKH/红色素浓缩激素超家族成员，pQEGLDHMFMRFamide，一种新型肌抑制素，以及 SYKSKPPENGSIFamide，SIFamide 家族的新成员。由于这里提供的肽是迄今为止唯一从暹罗A. siamensis或任何鳃类中描述的肽，因此它们代表了一种新的资源，可以开始研究肽能控制这种和其他相关水产养殖害虫的生理和行为。 (C) 2014 Elsevier Inc. 保留所有权利。
Recent advances in high-throughput sequencing have facilitated the generation of large transcriptomic datasets for an ever-growing number of crustaceans, one being the carp louse Argulus siamensis. This and other members of the subclass Branchiura are obligate fish ectoparasites, and as such, are a major concern for commercial aquaculture. Using the extant transcriptome shotgun assembly (TSA) sequences for A. siamensis, 27 transcripts encoding putative-neuropeptide-precursors were-identified, and their pre/preprohormones deduced and characterized using a well-established bioinformatics workflow. The structures of 105 distinct peptides were predicted from the deduced proteins, including isoforms of adipokinetic hormone (AKH), allatostatin A, allatostatin B, allatostatin C, allatotropin, bursicon alpha, bursicon beta, crustacean cardioactive peptide (CCAP), diuretic hormone 31, diuretic hormone 44, eclosion hormone, myosuppressin, neuroparsin, neuropeptide Y, orcokinin, pigment dispersing hormone, proctolin, short neuropeptide F, SIFamide, sulfakinin and tachykinin-related peptide. While several of the predicted peptides are known from other crustacean and/or insect species, e.g. RYLPT, a broadly conserved arthropod proctolin isoform, and PECNAFTGCamide (disulfide bridging between the two cysteines), the stereotypical crustacean CCAP, the vast majority of them are described here for the first time, e.g. pQVNESTKWamide, a new AKH/red pigment concentrating hormone superfamily member, pQEGLDHMFMRFamide, a novel myosuppressin, and SYKSKPPENGSIFamide, a new member of the SIFamide family. As the peptides presented here are the only ones thus far described from A. siamensis, or for that matter, any branchiuran, they represent a new resource to begin investigations of peptidergic control of physiology and behavior in this and other related aquacultural pests. (C) 2014 Elsevier Inc. All rights reserved.