Atrial Gene Painting in Large Animal Model of Atrial Fibrillation.
Atrial Gene Painting in Large Animal Model of Atrial Fibrillation.
复制标题
心房颤动大型动物模型中的心房基因绘制。
DOI：
10.1007/978-1-0716-2707-5_16
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Donahue,JKevin
中科院分区：
文献类型：
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作者：
Mo,Weilan;Donahue,JKevin
基因治疗似乎是心脏疾病的一种靶向治疗方法。房颤是最常见的持续性心律失常，也是导致中风、心力衰竭和死亡的主要因素。房颤的发生和维持机制与整个心房的结构和电生理重构有关。选择合适的基因传递方式是实现基因转导效果的关键。理想的治疗房颤的基因传递方法应该是在心房提供广泛和充分的转基因暴露，只有在安全地维持心脏的动态平衡而没有非靶标表达的情况下才是如此。所有这些要求都可以通过直接应用于心房心外膜表面的心房基因涂抹来实现。在这一章中，我们介绍了心房基因绘制的优势和实验方法，并将其应用于一个大型动物房颤模型。
Gene therapy appears promising as a targeted treatment of cardiac diseases. Atrial fibrillation (AF) is the most common sustained cardiac arrhythmia and also a major contributor to stroke, heart failure, and death. Mechanisms that initiate and sustain AF are associated with structural and electrophysiological remodeling in the whole atria. Selection of the appropriate gene delivery method is critical for transduction efficacy. The ideal gene delivery method to manage AF should provide widespread and sufficient exposure to the transgene in atria only that safely maintains the homeostasis of the heart without off-target expression. All these requirements can be achieved using atrial gene painting that is directly applied to the atrial epicardial surface. In this chapter, we present the advantages of atrial gene painting and the experimental method, as applied to a large animal model of AF.