DISTINCT PATTERNS OF EXPRESSION OF THE D-TYPE CYCLINS DURING TESTICULAR DEVELOPMENT IN THE MOUSE
DISTINCT PATTERNS OF EXPRESSION OF THE D-TYPE CYCLINS DURING TESTICULAR DEVELOPMENT IN THE MOUSE
复制标题
DOI：
10.1002/dvg.1020160209
复制
发表时间：
1995-01-01
期刊：
影响因子：
--
通讯作者：
WOLGEMUTH, DJ
中科院分区：
文献类型：
--
作者：
RAVNIK, SE;RHEE, K;WOLGEMUTH, DJ
三种 D 型细胞周期蛋白已被证明在许多分离的细胞类型和细胞系中差异表达，表明在特定细胞谱系的细胞周期调节中具有不同的作用。睾丸为研究参与细胞周期调节的基因提供了独特的机会，因为它包含有丝分裂和减数分裂的细胞以及几乎没有增殖活性的分化细胞。通过细胞周期蛋白 D1、细胞周期蛋白 D2 和细胞周期蛋白 D3 的 Northern 杂交分析，在成年小鼠睾丸中分别检测到 4.2 kb、6.8 kb 和 2.3 kb 的主要转录本。通过 Northern 杂交在睾丸中检测到细胞周期蛋白 D3 的额外 1.8 和 2.7 kb 转录本，但在其他组织中未检测到，并且这些转录本仅限于生殖细胞。对正常睾丸和生殖细胞缺陷睾丸的 Northern 和原位杂交分析显示出令人惊讶的结果，即细胞周期蛋白 D1 的表达模式与非分裂支持细胞中的表达一致。与完整睾丸相比，生殖细胞缺陷睾丸中的细胞周期蛋白 D2 水平似乎略有富集，但原位杂交分析并未揭示任何明显的细胞定位。同样令人惊讶的是，细胞周期蛋白 D3 表达在非分裂、单倍体、圆形精子细胞中最高。讨论了这些细胞周期蛋白在精子发生事件中的可能作用。 (C) 1995 Wiley-Liss, Inc.
The three D-type cyclins have been shown to be differentially expressed in a number of isolated cell types and cell lines, suggesting distinct roles in cell cycle regulation in particular cell lineages. The testis provides unique opportunities to study genes involved in cell cycle regulation, since it contains cells in both mitosis and meiosis as well as differentiated cells with little proliferation activity. Major transcripts of 4.2 kb, 6.8 kb, and 2.3 kb were detected in the adult mouse testis by Northern hybridization analyses for cyclin D1, cyclin D2, and cyclin D3, respectively. Additional transcripts of 1.8 and 2.7 kb were detected by Northern hybridization for cyclin D3 in the testis, but not in other tissues, and these transcripts were limited to germ cells. Northern and in situ hybridization analyses of normal and germ cell-deficient testes showed the surprising result that cyclin D1 was expressed in a pattern consistent with expression in the non-dividing Sertoli cells. Cyclin D2 levels appeared slightly enriched in germ cell-deficient testes as compared to intact testis, but in situ hybridization analysis did not reveal any distinct cellular localization. Also surprising was the observation that cyclin D3 expression was highest in the non-dividing, haploid, round spermatids. The possible roles of these cyclins in the events of spermatogenesis are discussed. (C) 1995 Wiley-Liss, Inc.