The liver as the site of C-reactive protein formation.
The liver as the site of C-reactive protein formation.
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DOI：
10.1084/jem.123.2.365
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发表时间：
1966-02-01
期刊：
影响因子：
--
通讯作者：
Hochwald GM
中科院分区：
文献类型：
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作者：
Hurlimann J;Thorbecke GJ;Hochwald GM
c反应蛋白（CxRP， CRP）的形成位点已经在兔、猴和人的组织中进行了研究。通过注射松节油、巴豆油、内毒素、副伤寒疫苗或肺炎球菌刺激家兔和猴子产生急性期蛋白。c14 -氨基酸在体外的掺入是通过用培养液制成的免疫电泳图的放射自显影证实的。结果发现，在测试的许多不同组织中，肝脏是唯一将c14 -赖氨酸和异亮氨酸纳入CxRP或CRP的组织。只有在各种刺激后16至24小时的肝脏是有效的；正常动物或刺激后3至9小时死亡的动物的肝脏没有产生可检测到的CxRP。注射部位的发炎肌肉没有显示c14 -氨基酸掺入到CxRP中。一些人体组织也被培养出来，一些肝脏培养物被发现含有标记的CRP。肝脏生成的CxRP或CRP总是伴随着c14 -氨基酸与其他血清蛋白结合的增强，但并非总是相反。
The site of formation of C-reactive protein (CxRP, CRP) has been studied with tissues from rabbits, monkeys, and human beings. Rabbits and monkeys were stimulated to produce the acute phase protein by injection of turpentine, croton oil, endotoxin, paratyphoid-typhoid vaccine, or pneumococci. C14-amino acid incorporation in vitro was demonstrated by means of autoradiography of immunoelectrophoretic patterns made with culture fluids. It was found that among many different tissues tested liver was the only tissue which incorporated C14-lysine and isoleucine into CxRP or CRP. Only livers taken 16 to 24 hr after various stimuli were active; livers from normal animals or from animals killed 3 to 9 hr after stimulation did not produce detectable amounts of CxRP. Inflamed muscle from the injection site did not show C14-amino acid incorporation into CxRP. Several human tissues were also cultured, and a few liver cultures found to contain labeled CRP. The formation of CxRP or CRP by the liver was always accompanied by enhanced C14-amino acid incorporation into other serum proteins, but the reverse was not always found.