The gel-forming polysaccharide of psyllium husk (Plantago ovata Forsk)
The gel-forming polysaccharide of psyllium husk (Plantago ovata Forsk)
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DOI：
10.1016/j.carres.2004.05.023
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发表时间：
2004-08-02
影响因子：
3.1
通讯作者：
Marlett, JA
中科院分区：
文献类型：
--
作者：
Fischer, MH;Yu, NX;Marlett, JA
通过组成分析、甲基化分析和核磁共振波谱分析，研究了车前草籽壳（车前子籽）碱提取多糖及其部分水解产物的生理活性和凝胶形成组分。解决了先前研究者的相互矛盾的说法，发现该材料是中性阿拉伯木聚糖（阿拉伯糖22.6%，木糖74.6%，摩尔基础;只有痕量的其他糖）。约35%的非还原性末端残基，多糖是高度支化的。这些数据与由密集取代的β-（1 -> 4）-连接的D-吡喃木糖残基主链组成的结构相容，其中一些在2位携带单个吡喃木糖侧链，另一些在3位携带具有序列L-Araf-α-（1 -> 3）-D-Xylp-beta-（1 -> 3）-L-Araf的三糖分支。甲基化和NMR数据以及分离出的多糖部分酸水解产物二糖3-O-β-D-吡喃木糖基-L-阿拉伯糖支持了该序列的存在。(C)2004爱思唯尔有限公司保留所有权利。
The physiologically active, gel-forming fraction of the alkali-extractable polysaccharides of Plantago ovata Forsk seed husk (psyllium seed) and some derived partial hydrolysis products were studied by compositional and methylation analysis and NMR spectroscopy. Resolving the conflicting claims of previous investigators, the material was found to be a neutral arabinoxylan (arabinose 22.6%, xylose 74.6%, molar basis; only traces of other sugars). With about 35% of nonreducing terminal residues, the polysaccharide is highly branched. The data are compatible with a structure consisting of a densely substituted main chain of beta-(1 --> 4)-linked D-xylopyranosyl residues, some carrying single xylopyranosyl side chains at position 2, others bearing, at position 3, trisaccharide branches having the sequence L-Araf-alpha-(1 --> 3)-D-Xy1p-beta-(1 --> 3)-L-Araf. The presence of this sequence is supported by methylation and NMR data, and by the isolation of the disaccharide 3-O-beta-D-xylopyranosyl-L-arabinose as a product of partial acid hydrolysis of the polysaccharide. (C) 2004 Elsevier Ltd. All rights reserved.