Validation of laser Doppler fluxmetry as a method of assessing neo-angiogenesis in laryngeal tumours
Validation of laser Doppler fluxmetry as a method of assessing neo-angiogenesis in laryngeal tumours
复制标题
DOI：
10.1007/s00405-005-1020-4
复制
发表时间：
2006-05-01
影响因子：
2.6
通讯作者：
Birchall, M. B.
中科院分区：
文献类型：
--
作者：
Jacob, A.;Varghese, B. E.;Birchall, M. B.
我们的目的是验证以下假设：激光多普勒通量测量能够有效地表示底层组织的血流，并为测量人类喉癌的血流提供可靠的工具。这是一项实验性前瞻性研究。我们对 20 名喉癌患者进行了激光多普勒通量测定，并将通过该方法获得的值与活检中手动计数的微血管密度（公认的金标准）进行了比较。我们在微血管密度的估计中表现出良好的观察者间和观察者内变异性。然后，我们将微血管密度与激光多普勒通量测定法进行比较，并使用 Spearman 检验来评估非参数相关性，我们得到的相关系数为 0.4（P 值：0.15）。据我们所知，这项研究首次证明了与激光多普勒通量测定法相比，传统测量的血管生成之间存在积极趋势。
Our intention was to validate the hypothesis that laser Doppler fluxmetry would give a valid representation of underlying tissue blood flow and would provide a reliable tool for measuring blood flow in human laryngeal cancers. This was an experimental prospective study. We performed laser Doppler fluxmetry on 20 patients with laryngeal cancer and compared the values obtained through this method with manual counting of microvessel density from biopsies, the accepted gold standard. We demonstrated good inter- and intraobserver variability in our estimation of microvessel density. We then compared microvessel density with laser Doppler fluxmetry, and using Spearman's test to assess nonparametric correlation, we obtained a correlation coefficient of 0.4 (P value: 0.15). To our knowledge, this study is the first of its kind to demonstrate a positive trend between angiogenesis measured conventionally when compared with laser Doppler fluxmetry.